Mixed DNA

Question 1

Type A

Answer 1

alle peaks same height

Question 2

Type B

Answer 2

two very distinguishable profiles

Question 3

Type C

Answer 3

such weak signal that you might miss alleles

Question 4

Steps for interpreting mixtures

Answer 4

1- identify presence of mixture
2- designate allele peaks (which major, which minor)
3- identify the number of potential contributors
4- estimate the relative ratio of the individuals contributing to the mixture
5- consider all possible genotype combinations
6- compare reference samples

Question 5

Differentiate major/minor component

Answer 5

easiest to start with locus with maximum number of alleles
use that to estimate relative ratio- one person to another
determine genotype at other loci
determine RMP of each separate genotype

Question 6

Challenges of differentiation

Answer 6

stutter peaks
roughly equal contributions
little DNA- stochastic effects

Question 7

PE

Answer 7

probability of exclusion
proportion of population that has a least one allele that was NOT observed
easiest to calculate based on probability of inclusion

Question 8

LR

Answer 8

likelihood ratio

ratio of probabilities- Hp/Hd