Smiley-7: Negative and dsRNA Expression and Replication Flashcards
What must -ve and dsRNA do before any protein translation?
transcribe their genome
-ve strand RNAs are divided into 2 groups:
- monopartite genome
- multipartite genome
VSV (vsicular stomatitis virus) genome description (x3)
multipartite or monopartite?
- monopartite
- -ve strand
- RNA
How many genes does VSV have in its genome?
5
What proteins comprise the RdRNAP of VSV?
L and P proteins
How does VSV create polyA tail?
stuttering mechanism
How does VSV make a full-length + strand genome?
N protein coats -ve strand genome to prevent polyadenylation and termination reactions
How is paramyxo virus’ ORF different from others?
some copies of mRNA encoding V protein are edited by adding extra G residues to change reading frame to make another mRNA encoding a different protein
How does “editing” occur in paramyxo viruses?
stuttering in a run of C’s in template strand during transcription
Describe influenza’s genome (x3)
multipartite or monopartite?
- multipartite
- ss
- -ve sense RNA
How many RNA segments are present in influenza genome?
8
Where does influenza genome go to get transcribed?
host’s nucleus
How does influenza acquire 5’ cap?
its RdRNAP cleaves 5’ cap of host cell and attaches it to the -ve sense template
How is influenza virus polyadenylated?
stuttering reaction
What mediates the switch from transcription to replication in influenza?
NP protein
