Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

IMIN 324 > Marchant-15: Immune Response to Viral Infection I > Flashcards

Marchant-15: Immune Response to Viral Infection I Flashcards

1
Q

What are the 2 pathways stimulated by IFN to stimulate antiviral state?

A
  • oligoadenylate synthetase (OAS1)

- Protein kinase R (PKR)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What upregulates OAS1?

A

IFN

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What activates/alerts OAS1 activation?

A

dsRNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Function of OAS1

A

polymerizes ATP into 2’-5’ ATP oligomers

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Where does 2’-5’ ATP oligomers bind?

A

endoribonuclease L

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What does the OAS1 pathway accomplish?

A

degradation of ssRNA (viral and cellular)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Where is PKR normally found?

A

cytoplasm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What upregulates PKR?

A

IFN

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What activates PKR?

A

dsRNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Goal of PKR

A

block viral and cellular protein translation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What does PKR phosphorylate to inhibit protein translation?

A

eIF-2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What does adenovirus use to inhibit PKR action?

A

VA-RNA1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

How does VA-RNA1 inhibit PKR?

A

binds to PKR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What do poxviruses produce to inhibit IFN action?

A

M-T7

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Function of MT7

A
  • homologous to IFN receptor

- binds to cellular IFN receptor and prevents interaction with cell receptor

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What does vaccinia produce to inhibit PKR? This has a RNA binding domain.

A

E3L

17
Q

What does vaccinia produce that inhibits PKR and has homology to elF-2?

A

K3L

18
Q

Function of K3L

A

has homology to elF-2 which prevents it from phosphorylating = blocks its action and allows translation of proteins

19
Q

Function of calnexin

A

chaperone that helps MHC I alpha and beta-2-M chains assemble in ER

20
Q

What does adenovirus express that aids in localization of virus to ER?

A

E3-19K

21
Q

How is adenovirus retained in ER?

A

it has ER retention sequence (Lys-rich)

22
Q

What is the function of E3-19K?

A

binds MHC I heavy chain –> inhibits expression of MHC I on cell surface

23
Q

What does herpes virus produce to inhibit MHC I expression on cell surface?

A

ICP47

24
Q

What is the function of ICP47?

A

blocks transport of peptides into ER

25
Q

What does HCMV produce that inhibits MHC I expression at cell surface?

A
  • US6-ER
  • Us3
  • Us11 and Us2
26
Q

How does US6-ER work?

A

prevents peptides from entering ER

27
Q

How does Us3 work?

A

retains MHC in ER

28
Q

How does Us11 work?

A

translocate MHC I to cytoplasm where it degrades

29
Q

2 mechanisms of CD8 T cells in killing virus infected cells

A
  • release of cytotoxic granules to target cell

- ligates Fas ligands on virus infected cell –> apoptosis

30
Q

Fas and what other ligand are signals for apoptosis?

A

TNFR1

31
Q

3 virus mechanisms to inhibit Fas and TNF killing?

A
  • Caspase 8 inhibitors
  • DED
  • Downregulation of Fas receptors from cell surface

IMIN 324 (14 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions