Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

IMIN 324 > Smiley-2 > Flashcards

Smiley-2 Flashcards

1
Q

Relationship between viral genome size and protein coding capacity

A

The bigger the genome, the more protein it can encode

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

+ sense strand

A

Same sequence as protein-coding mRNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Baltimore Classification

A

Classification of viruses based on how they make mRNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What type of forces join capsid subunits together?

A

Non covalent forces

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

2 types of capsid symmetry

A

Icosahedral and helical

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What dictates the length of the TMV virion?

A

The size of genome. Longer genome, longer virion (helical)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

icosahedron specs

A
  • 6-fold symmetry on each face

- 5-fold symmetry on each vertex

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What T numbers has pentons and hexons

A

T>1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Penton

A
  • groups of 5 subunits

- make up the 12 vertices

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Hexon

A
  • groups of 6 subunits

- make up the faces

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

When T>1, how many penton subunits are there?

A

always 60

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

How to find out number of hexon subunits for T>1?

A

60T-60

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Give examples of viruses with dsDNA genomes

A

herpesvirus, bacteriophage T4

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Examples of viruses with ssDNA genomes?

A

parvovirus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What complexes do DNA viruses need to make mRNA and duplicate genome?

A
  • DNA-dependent DNAP

- DNA-dependent RNAP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Examples of viruses with dsRNA

A

reovirus

17
Q

What complex do RNA viruses need to make mRNA and duplicate genome?

A
  • RNA-dependent RNAP
18
Q

examples of + sense ssRNA

A

poliovirus, Zika virus

19
Q

examples of - sense ssRNA

A

influenza, measles

20
Q

examples of retroviruses

A

HIV

21
Q

ICTV scheme of classification

A

takes into account properties of virus particle, genetic relationships, genome structure, plus mRNA generation

22
Q

Most DIRECT method of detecting and quantifying virus particles

A

Electron microscopy (EM)

23
Q

Downfall of EM

A

Only gives the particle count and not the proportion of particles that are infectious

24
Q

INDIRECT methods of detecting and quantifying viral genome (x2)

A
  • nucleic acid hybridization

- PCR

25
Q

What technique can detect viral structural proteins using specific antibodies?

A

Immuno-detection assays.

26
Q

Technique used to detect infectivity

A

Plaque assay

27
Q

Particle-to-PFU ratio

A

how many virus particles does it take to cause one plaque

28
Q

Tissue Culture Dose 50 (TCD50)

A

Dilution resulting in half the cultures receiving a single plaque

29
Q

T or F: During infection, viral infectivity disappears when the viral genome is released from capsid and does not reappear until progeny virions are assembled.

A

True

30
Q

Multiplicity of Infection (MOI)

A

How many virions are added per cell

IMIN 324 (14 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions