Skin And Soft Tissues

Question 1

Laboratory diagnosis of gas gangrene

Answer 1

LABORATORY DIAGNOSIS
SECTION 7 • Skin, Soft Tissue and Musculoskele
* Swabs ru are not s
* Specime broth an
Gas gangrene
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Specimen: Necrotic tissues, muscle fragments and exudates from deeper parts of the wound
• Direct microscopy: Thick, stubby, boxcar-shaped gram-positive bacilli without spore are suggestive of C. perfringens
口 Culture：
Media: Robertson cooked meat broth, egg yolk agar, etc.
> Incubation: Anaerobically (by GasPak or Anoxomat, etc.)
Identification of C. perfringens
> Target hemolysis (double zone hemolysis)
> Nagler’s reaction: Opalescence surrounding the streak line on egg yolk agar
Reverse CAMP test: Positive
>
Heat tolerance test: Positive
In litmus milk: Produces stormy clot reaction.

Question 2

Laboratory diagnosis of VZW

Answer 2

Laboratory Diagnosis (VZV)
Laboratory diagnosis of VZV infection is as follows:
Specimen collection: Common specimens include vesicular lesions, scabis and maculopapular lesions
* Cytopathology: Giemsa staining of the scrapings from the ulcer base (Tzanck smear) reveals cytopathological of multinucleated giant celli
changes similar to that of HSV infection,

Question 3

Drugs for VZW?

Answer 3

Valocyclovir
Acyclovir
Famiciclovir

Question 4

Incubation period of VZW.
Main clinical manifestation?

Answer 4

A.2-3weeks
B.rashes

Question 5

What is causative agent of gangrene?
Classification of clostridium
Causative agent? A. Established agents
B.probable agents

Question 6

Pathogene sis of gas gangrene?

Question 7

Laboratory diagnosis of has gangrene

Answer 7

• Specimen: Necrotic tissues, muscle fragments and exudates
species
from deeper parts of the wound
Citror
• Direct microscopy: Thick, stubby, boxcar-shaped gram-positive
larly s
bacilli
without spore are suggestive of C. perfringens
• Large
■ Culture：
С. пог
Media: Robertson cooked meat broth, egg yolk agar, etc.
> Incubation: Anaerobically (loy GasPak or Anoxomat, etc.)
Identification of C. perfringens
› Target hemolysis (double zone hemolysis)
> Nagler’s reaction: Opalescence surrounding the streak line on egg yolk agar
> Reverse CAMP test: Positive
Heat tolerance test: Positive
In litmus milk: Produces stormy clot reaction.