CNS

Question 1

Laboratory diagnosis of Rabies

Answer 1

• Antigen detection from hair follicles at nape of the neck and from corneal smear—by direct IF test
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Viral Isolation by:
Mouse inoculation
Cell lines inoculation-Mouse neuroblastoma and
EAK cell
lines.
Antibody detection from serum and CSF-by MNT, RFFI, FAVN, and IFA
Viral RNA detection—by RT-PCR
• Negri body detection in histopathological staining of brain biopsies (hippocampus)-for postmortem diagnosis of rabies.

Question 2

Prions disease : Classification . CODE ?
Further classification of CJD ?

Question 3

JE