Skildog HIV Flashcards
Capsid symmetry for HIV?
Icosahedral
Genome classification for HIV?
2 copies of + sense RNA
Enveloped?
Yes
What two envelope proteins are important for viral recognition?
gp41 and gp120
What is the tropism for HIV?
CD4+ T cells
Macrophages
Monocytes
What gene encodes the capsid?
Gag
What protein spikes early on in infection?
p24
What gene encodes for gp120 and gp41?
Env
____ is the glycoprotein that is not connected to the membrane on its own, but rather via ____ glycoprotein.
gp120, gp41
What two receptors are important for viral entry into T cells?
CD4 Chemokine receptors (CXCR4, CCR5)
What protein binds to CXCR4 to prevent viral entry into the cell?
SDF-1 (stromal derived factor)
What are the two functions of viral reverse transcriptase?
Make a ssDNA copy of viral RNA
Make dsDNA from ssDNA
What enzyme is responsible for the formation of a provirus from the viral dsDNA?
Integrase
What is the role of Tat?
Creates spliced RNA from genome (Replication machinery)
What is the role of Rev?
Creates all of the products that will be incorporated into the final virus particle
What is the role of Nef?
Promotes budding of virus out of T cell
What important protein for recognition is encoded by Gag?
p24
What three important proteins are encoded by Pol?
Reverse transcriptase, protease, integrase
What are LTR’s
Integration sites important for incorporation into genome,
Strong promoters of transcription
What two testing techniques are used for monitoring HIV progress in patients?
CD4+ count via flow cytometry
PCR of vDNA or vRNA
