Simple Staining and Negative Staining

Question 1

The portion of the chromogen that gives it color is called the

Answer 1

Chromophore

Question 2

The charged portion of a chromogen is known as the

Answer 2

Auxochrome

Question 3

Stains consist of a ______ , and a colored molecule called the _____

Answer 3

Solvent;Chromogen

Question 4

The two common dyes are

Answer 4

Basic Dyes and Acidic Dyes

Question 5

Basic dyes have a _____ charge and stain the_____

Answer 5

Positive; Cells

Question 6

Acidic dyes have a ______ charge and stain the____

Answer 6

Negative; Background

Question 7

Why are acidic dyes repelled by bacterial cell membranes?

Answer 7

Because bacterial cells also have a negative charge

Question 8

Simple stains use what type of dye?

Answer 8

Basic Dye

Question 9

Simple stains show these three physical characteristics

Answer 9

Size, Shape and Arrangement

Question 10

Circular shaped bacterial cells are

Answer 10

Coccus

Question 11

Rod shaped bacterial cells are

Answer 11

Bacillus

Question 12

Pairs of cells form this arrangement

Answer 12

Diplo

Question 13

Irregular clusters form this arrangement

Answer 13

Staphylo

Question 14

Chains of cells form this arrangement

Answer 14

Strepto

Question 15

What three things does heat fixation accomplish

Answer 15

1. It kills the organisms 2. It cause the organism to adhere to the slide 3. It alters the organism so that they can more readily accept stains

Question 16

When are negative stains useful

Answer 16

To determine morphology and cellular arrangement in bacteria that are to delicate to withstand heat fixing.

Question 17

Negative staining requires which type of dye

Answer 17

Acidic Dye

Question 18

What gets stained during a negative staining procedure

Answer 18

The background