Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Davis BIO 225 (Lab) > Selective and Differential Tests > Flashcards

Selective and Differential Tests Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

A media that encourages the growth of some organisms but suppresses the growth of others

A

Selective Media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

This media causes an observable change (color or change in pH) in the medium when a particular biochemical reactions occurs. So most bacteria will grow, but they will grow different colors based on their interaction with the media

A

Differential Media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

This media contains special nutrients that allow growth of a particular organism that might not otherwise be present in sufficient numbers.

A

Enrichment Media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Used for the isolation and differentiation of Staphylococcus Aureus

A

Mannitol Salt Agar (MSA)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What makes the MSA agar selective

A

Sodium Chloride (Salt)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Staphylococcus bacteria thrive in high salt concentrations. But what happens to other bacteria such a E.coli

A

They Dehydrate (shrivel) and die

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What makes the MSA agar differential

A

The Mannitol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

If Staphylococcus bacteria ferment the Mannitol what color will the growth be

A

Yellow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

If Staphylococcus bacteria do not ferment the Mannitol what color will the growth be

A

Pink

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

When Mannitol is fermented what happens to the pH in the media

A

Because acids are released the pH goes down

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Used to isolate and differentiate members of enterobacteriaceae based on the ability to ferment lactose

A

MacConkey Agar (MAC)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What makes the MAC agar selective

A

Bile salts and crystal violet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

In MAC agar what type of bacteria are inhibited from growing

A

Gram positive bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

In MAC agar what makes the media differenital

A

The lactose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

When bacteria such as E.coli ferment lactose what color is the growth on a MAC agar plate

A

Pink

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Used for the isolation of fecal coliforms

A

Eosin Methylene Blue Agar (EMB)

17
Q

What sugars are present in an EMB agar plate

A

Sucrose and Lactose

18
Q

What type of bacteria are inhibited from growing on an EMB plate

A

Gram positive bacteria

19
Q

What components of the EMB plate make it selective

A

Eosin Y Dye and Methylene Blue

20
Q

What type of bacteria typically grow with a metallic green sheen on an EMB plate?

A

E.coli

21
Q

What color will the bacteria grow on an EMB plate if they ferment sucrose?

A

They retain the color of the medium.

22
Q

What color will the bacteria grow on an EMB plate is they ferment lactose?

A

Depending on the bacteria it could be dark purple, black or metallic green.

23
Q

The citrate test is used to determine the ability of an organism to use citrate as its sole source of _____

A

Carbon

24
Q

The enzyme _______ will transport citrate to the cell and perform citrate fermentation

A

Citrate Permease

25
Q

Will the pH of the Citrate media increase or decrease after citrate has been utilized

A

Increase

26
Q

A citrate positive test will turn which color (assuming you add enough bacteria)

A

Blue

27
Q

During a urea test you are testing bacteria to see if they have what enzyme?

A

The enzyme urease

28
Q

How long do you have to read the results of a urea test?

A

24 hours

29
Q

Will the pH of the media increase or decrease when urea is broken down?

A

Increase

30
Q

What color is a positive urea test?

A

Pink

31
Q

Allows for differentiation of bacteria based on their ability to hemolyze RBCs

A

Blood Agar

32
Q

The complete destruction of RBCs and hemoglobin, results in a clear growth around the colonies

A

Beta Hemolysis

33
Q

The partial destruction of RBCs and produces a greenish discoloration of agar around the colonies.

A

Alpha Hemolysis

34
Q

Non-hemolysis and appears as simple growth with no change to the medium.

A

Gamma Hemolysis

Davis BIO 225 (Lab) (7 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions