Acid Fast and Endospore Stain

Question 1

A differential stain which distinguishes organisms with waxy cell walls that can resist decolorization with acid alcohol.

Answer 1

Acid Fast Stain

Question 2

The acid fast stain identifies bacteria in the Genus

Answer 2

Mycobacterium

Question 3

A waxy substance that gives acid-fast cells a higher affinity for the primary stain and resistance to the decolorization by an acid alcohol solution.

Answer 3

Mycolic acid

Question 4

Acid fast positive bacteria stain which color

Answer 4

Pink

Question 5

Acid fact negative bacteria stain which color

Answer 5

Blue

Question 6

The correct order of the reagents for an acid fast stain is

Answer 6

Carbolfuchsin, acid alcohol and Methylene blue

Question 7

In the acid fast stain which dye is the primary stain?

Answer 7

Carbolfuchsin,

Question 8

In the acid fast stain which dye is the counterstain?

Answer 8

Methylene Blue

Question 9

What is the decolorizing agent called in the acid fast stain?

Answer 9

Acid Alcohol

Question 10

A dormant form of the bacterium that allows it to survive poor environmental conditions

Answer 10

Endospore

Question 11

Spores are resistant to heat and chemicals because of a tough outer covering made of the protein

Answer 11

Keratin

Question 12

Two most common genera that produce spores

Answer 12

Bacillus and Clostridium

Question 13

Metabolically active bacterial cells are know as

Answer 13

Vegetative cells

Question 14

Vegetative cells will stain which color during an endospore stain

Answer 14

Pink

Question 15

Endospores will stain which color during an endospore stain

Answer 15

Green

Question 16

What is the correct order of reagents used during an endospore stain

Answer 16

Malachite green, water and Safranin