Biochemical Tests Flashcards
The superoxide ion is first converted to hydrogen peroxide and normal oxygen by the action of an enzyme called
Superoxide dismutase.
Hydrogen peroxide must be degraded by an enzyme called _____ into water and oxygen
Catalase
A catalase positive test will have what reaction?
It will bubble
Used to identify bacteria containing the respiratory enzyme cytochrome c oxidase
Oxidase Test
An oxidase positive test will turn what color
Blue
What is the time limit for interpretation of an Oxidase test
20 Seconds
The SIM medium is used for determination of which three bacterial activities
Sulfur Reduction, Indole Production and Motility
During a SIM test how will you know that sulfur reduction occurred?
The media will turn black
During a SIM test if a thick stab line is present than your bacteria most likely have a______
Flagella
Tryptophan is broken down into indole and pyruvate by which enzyme?
The tryptophanase enzyme
What is the name of the reagent used to check for indole production?
Kovacs Reagent
Indole is a by product of the break down of what?
Tryptophan
Some organisms float on top of the medium and produce a type of surface membrane called a
Pellicle
Some organisms sink to the bottom of a nutrient broth. This is called a
Sediment
Growth at the top around the edges of a nutrient broth is known as a
Ring
