Simple Stain Flashcards
1
Q
A simple stain uses only 1 ____
A
Dye or chromophore
2
Q
What charge does chromophore have?
A
Positive
3
Q
What charge does a cell have?
A
Negative
4
Q
What information is provided by this type of stain
A
Size, shape, arrangement
5
Q
What step follows heat fixing?
A
Cover smear with methylene blue for 30-60 seconds
6
Q
How many drops of methylene blue are added?
A
5-6
7
Q
What do you do after methylene blue?
A
Wash slide carefully with distilled water
8
Q
How is slide held during rinsing
A
Tilted, water aimed above smear
9
Q
What follows rinsing step?
A
Blot slide dry
10
Q
When finished viewing slide with microscope, what needs to be done?
A
Gently blot oil from the slide and store it in a slide box
