Gram Stain Procedure

Question 1

Step 1

Answer 1

Gently flood smears with crystal violet and let stand for 1 minute

Question 2

Step 2

Answer 2

Gently wash with tap water

Question 3

Step 3

Answer 3

Gently flood smears with grams iodine, let stand for 1 minute

Question 4

Step 4

Answer 4

Gently wash with tap water

Question 5

Step 5

Answer 5

Decolorize with ethanol alcohol (drop by drop until alcohol runs almost clear)

Question 6

Step 6

Answer 6

Gently wash with tap water

Question 7

Step 7

Answer 7

Counterstain with safranin

Question 8

Step 8

Answer 8

Gently wash with tap water

Question 9

Step 9

Answer 9

Blot dry with bibulous paper. Examine under oil immersion