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Micro > Gram Stain > Flashcards

Gram Stain Flashcards

1
Q

What is gram stain used for

A

Identifying bacteria and staining them

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2
Q

How are bacteria distinguished with the gram stain?

A

Cell wall

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3
Q

What age of Cell/culture gives the best gram stain result?

A

Cells less than 24 hours old

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4
Q

Once smear is complete what is the first step of the gram stain? (What is added)

A

Crystal violet

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5
Q

When times up after adding crystal violet what is done?

A

Rinse with distilled water

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6
Q

How long does the first reagent sit in the slide? What is time in lab manual?

A

30 seconds.

1 minute in lab manual

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7
Q

How do the times differ with the second reagent in the video compared to lab manual?

A

10 seconds to 1 minute in video.

1 minute in lab manual

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8
Q

What reagent functions to decolorize?

A

Ethanol

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9
Q

What happens if too much alcohol is added to the slide?

A

Crystal violet can be rinsed from gram positive bacteria

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10
Q

How specifically is alcohol added to the slide?

A

Drop by drop

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11
Q

What is the last step of gram stain? (What’s added)

A

Safranin

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12
Q

What color results if the cells have thick layer of peptidoglycan? They are gram _____

A

Purple

Positive

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13
Q

What color results if the cells have a thin layer of peptidoglycan? They are gram ___

A

Pink

Negative

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14
Q

What is step 2 of the gram stain procedure? (What is added)

A

Grams iodine

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15
Q

When the time is up after adding grams iodine what do you do next?

A

Wash the slide by letting water wash over the smear until water runs clear

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16
Q

What follows the decolorization process?

A

Rinse with water

17
Q

How do the decolorization times in the video differ from the lab manual?

A

Video says do it until no large amounts of purple wash out

Manual says do it until alcohol runs clear showing no blue tinge

18
Q

What is done when the time is up after safranin?

A

Rinse with water

19
Q

What do you do following rinsing of safranin?

A

Use a Kim wipe to blot slide dry

Micro (8 decks)

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