Session 8 (Gene Expression) Flashcards

1
Q

What are the 3 stages of gene expression?

A

Initiation, elongation and termination

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2
Q

What does a transcription factor do?

A

Tells RNA polymerase to bind at specific points

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3
Q

What is initiation?

A

When RNA polymerase binds to DNA

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4
Q

What is elongation?

A

5’ to 3’ chain growth

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5
Q

What is formed during elongation?

A

A pre RNA molecule

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6
Q

What happens during transcription termination?

A

Termination sequence is reached, pre-RNA molecule=released

Cut by endonucleases

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7
Q

What are the 3 forms of RNA processing?

A

Capping, tailing, splicing

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8
Q

What is capping?

A

A 5’-5’ linkage which protects against degradation

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9
Q

What is tailing?

A

A polyA tail is added to the 3’ end- protects against degradation

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10
Q

What enzyme does the tailing?

A

PolyA polymerase

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11
Q

What is splicing?

A

Introns (different combinations of) being removed

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12
Q

How much + how many kinds of rRNA, mRNA and tRNA are there?

A

rRNA-few kinds but many copies
mRNA-many kinds but few copies
tRNA- many kinds and many copies

This sort of thing is easier to get your head around if you think about the jobs that each of the RNA does

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13
Q

What type of ribosomes do eukaryotic cells have?

A

60s+20s=80s

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14
Q

If the genetic code is read 5’ to 3’, what way are proteins produced? (N or C end first)

A

N terminal first

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15
Q

What is the first amino acid in every protein?

A

Methionine (AUG)

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16
Q

What are the 3 stop codons?

A

UAA, UAG, UGA

17
Q

What is a stem loop?

A

H bonds formed between Antiparallel sequences

18
Q

What is the base that recognises A, U + C?

A

Inosine

19
Q

What are the benefits of having inosine as a base in an anticodon?

A

It speeds up protein synthesis

20
Q

What happens during translation initiation?

A

mRNA binds to ribosome, AUG recognised

21
Q

What is tRNA called when it is attached to an amino acid?

A

Aminoacyl tRNA

22
Q

How are amino acids joined together during translation?

A

Translocation, amino acid on P site moved on top of amino acid on A site

(P site on left)*

23
Q

What enzyme is responsible for translocation?

A

Peptidyl transferase