Session 8 (Gene Expression) Flashcards
What are the 3 stages of gene expression?
Initiation, elongation and termination
What does a transcription factor do?
Tells RNA polymerase to bind at specific points
What is initiation?
When RNA polymerase binds to DNA
What is elongation?
5’ to 3’ chain growth
What is formed during elongation?
A pre RNA molecule
What happens during transcription termination?
Termination sequence is reached, pre-RNA molecule=released
Cut by endonucleases
What are the 3 forms of RNA processing?
Capping, tailing, splicing
What is capping?
A 5’-5’ linkage which protects against degradation
What is tailing?
A polyA tail is added to the 3’ end- protects against degradation
What enzyme does the tailing?
PolyA polymerase
What is splicing?
Introns (different combinations of) being removed
How much + how many kinds of rRNA, mRNA and tRNA are there?
rRNA-few kinds but many copies
mRNA-many kinds but few copies
tRNA- many kinds and many copies
This sort of thing is easier to get your head around if you think about the jobs that each of the RNA does
What type of ribosomes do eukaryotic cells have?
60s+20s=80s
If the genetic code is read 5’ to 3’, what way are proteins produced? (N or C end first)
N terminal first
What is the first amino acid in every protein?
Methionine (AUG)