Session 6 (Enzymes) Flashcards
What is the transition state?
The high energy intermediate that lies between substrate and product
What bonding holds the enzyme substrate complex together?
Hydrogen, ionic, hydrophobic interactions
What is V0?
Initial rate of reaction
What is Km?
The [substrate] at which half of the enzymes are saturated (1/2 Vmax)
What is Vmax?
Max rate at which all enzyme active site are saturated
What shape is the graph that shows the relationship between [substrate] and enzyme saturation?
Retangular hyperbola
What does a high Km mean?
The enzyme has low affinity for substrate
What does a low Km mean?
The enzymes has a high affinity for the substrate
What is a unit?
The amount of enzyme that produces 1 um of products per min under standard conditions
How can a unit of enzyme be standardised?
By adding ‘per L of serum’ or ‘per g of tissue’ after it
Ie 2 units per L of serum
What other graph can be used to get values for Km and Vmax?
A double reciprocal
How do you read Km and Vmax values from a lineweaver Burk plot?
X intercept= -1/Km
Y intercept= 1/Vmax
When looking at a double reciprocal graph when is Km bigger?
The closer the x intercept gets to x=0
When looking at a double reciprocal graph, when is Vmax bigger?
When the y intercept is closer to y=0
How do enzymes work?
By lowering the Ea of a reaction
