Session 2 (Intro To DNA)

Question 1

What is a nucleotide?

Answer 1

Sugar+base+phosphate group

Question 2

What is a nucleoside?

Answer 2

Sugar + base

No phosphate

Question 3

What happens if there is no OH on the 3rd carbon?

Answer 3

Elongation ceases

Question 4

What is the difference between ribose and deoxyribose?

Answer 4

Ribose= OH on carbon 2
Deoxyribose= H on carbon 2 (lacking an O)

Question 5

How many hydrogen bonds are formed between G+C, A+T, A+U?

Answer 5

2 between g and c

3 between A+U, A+T

Question 6

What bonds do nucleotides makes?

Answer 6

Phosphodiester bonds

Question 7

Why do H bonds form in DNA?

Answer 7

There. Is slight polarity in the bases

Question 8

What direction does DNA polymerase read the template strand?

Answer 8

3’->5’

Question 9

What is the origin of replication?

Answer 9

Particular sequence in genome at which replication is initiated

Question 10

What is needed to start initiation?

Answer 10

Primase

Question 11

What strands do you get during elongation? And why?

Answer 11

Leading and lagging strands

Lagging strand consists of okazaki fragments because the DNA polymerase is reading the template strand in a 3’-5’ direction

Question 12

How are the okazaki fragments repaired?

Answer 12

Ligase enzymes