Session 12: Molecular Techniques

Question 1

Name 3 molecular analysis techniques for DNA.

Answer 1

1. PCR
2. Gel electrophoresis
3. Restriction enzymes

Question 2

Name 3 molecular analysis techniques for proteins.

Answer 2

1. Enzyme assays
2. Protein electrophoresis
3. Immunoassays

Question 3

TRUE OR FALSE: only bacteria produce endonucleases

Answer 3

TRUE

Question 4

Endonucleases are mostly ________ of 4,5,6,8 bp.

Answer 4

Palindromes

Question 5

TRUE OR FALSE: primers should be in the same direction

Answer 5

FALSE: one forwards and one reverse primer is required

Question 6

How can proteins be separated in protein electrophoresis?

Answer 6

1. Size
2. Shape
3. Charge

Question 7

What is the concept behind SDS-PAGE?

Answer 7

Proteins are denatured, breaking the 3D structure hence creating a single polypeptide chain which allows the separation of proteins by size.

Question 8

How does isoelectric focusing (IEF) separate proteins?

Answer 8

By charge: proteins migrate until they reach there pI

Question 9

What does ELISA stand for?

Answer 9

Enzyme linked immunoabsorbant assay

Question 10

What does an ELISA show?

Answer 10

The concentration of proteins present in solution using antibodies.

Question 11

TRUE OR FALSE: during an ELISA the rate of colour formation is proportional to the amount of specific antibody

Answer 11

TRUE