Session 8: Gene Expression

Question 1

Name 3 processes which are required to convert pre-mRNA to mature mRNA.

Answer 1

1. 5’ Capping
2. Poly A tail (polyadenylation)
3. Splicing

Question 2

Why is RNA processing required?

Answer 2

To prevent mRNA degradation.

Question 3

What link is formed within 5’ capping?

Answer 3

5’ to 5’

Question 4

What are ribosomes comprised of?

Answer 4

rRNA and proteins

Question 5

Name the three types of RNA

Answer 5

1. rRNA
2. mRNA
3. tRNA

Question 6

rRNA: How many kinds? How many copies?

Answer 6

• few kinds

- many copies

Question 7

mRNA: How many kinds? How many copies?

Answer 7

• 100,000s kinds (due to splicing)

- few copies

Question 8

tRNA: How many kinds? How many copies?

Answer 8

• 100s kinds

- many copies

Question 9

Name 4 features of the genetic code.

Answer 9

1. Triplet code
2. Degenerate
3. Non-overlapping
4. Comma-less

Question 10

What is the aa initiation sequence?

Answer 10

AUG

Question 11

What are the 3 aa termination sequences?

Answer 11

UAA
UAG
UGA

Question 12

How are stem loops formed in a tRNA clover structure?

Answer 12

Hydrogen bonds form between anti-parallel complementary sequences.

Question 13

What is the anticodon triplet code?

Answer 13

5’ CAU 3’

Question 14

What is wobble base pairing?

Answer 14

A wobble base pair is a pairing between two nucleotides in RNA molecules that does not follow Watson-Crick base pair rules.