Session 12 ILO's Molecular Techniques

Question 1

Explain how standard molecular processes such as gene cloning, and restriction analysis work

Question 2

Explain the principles, theory and application of DNA electrophoresis

Question 3

Explain the principles, theory and application of protein gel electrophoresis

Question 4

Outline how DNA is sequenced

Question 5

Explain the molecular basis for PCR and design PCR primers to amplify DNA sequences

Question 6

Describe the basic theory behind PCR and appreciate its fundamental importance in genetic testing

Question 7

Describe the molecular basis of DNA hybridisation

Question 8

Describe protein electrophoresis

Question 9

Explain the process of Southern blotting and interpet simple blots

Question 10

Explain how microarray technology can be used to study gene expression and genome varation

Question 11

Outline applications of PCR and DNA hybridisation

Question 12

Understand the basis and use of enzyme assays

Question 13

Explain how antibodies can be used to detect proteins, including Western blotting

Question 14

Outline applications of other hybridisation - based technologies

Question 15

Explain the molecular basis of PCR

Question 16

Design appropriate primers to amplify a given DNA
sequence

Question 17

Interpret PCR analyses used to identify common genetic diseases

Question 18

Understand the molecular basis of nucleic acid hybridisation

Question 19

Outline the use of RT-PCR and the appropriate controls that should be used

Question 20

Explain the principles behind agarose gel electrophoresis

Question 21

Outline the mechanism of Sanger sequencing

Question 22

Outline the basic principles of SDS-PAGE

Question 23

Interpret simple SDS-PAGE gels to identify the molecular mass of proteins