Session 1: Method of studying tissues and the body

Question 1

Commonly used stains?

Answer 1

Hematoxylin and eosin

Question 2

Define basophilia.

Answer 2

The reaction of anionic cellular components and tissue with basic stains.

Question 3

Seven steps in tissue preparation.

Answer 3

1. Collect tissue
2. Add a fixative
3. Embed
4. Staining
5. Cut sections
6. Mount on microscope plate
7. Investigate with a microscope

Question 4

Define resolving power/resolution.

Answer 4

The ability to produce separate objects from items that are closely positioned.

Question 5

List 3 basophilic organelles.

Answer 5

1. Heterochromatin & nucleoli
2. Cytoplasmic components e.g ergastoplasm
3. Extracellular materials e.g carbs of matrix of cartilage