Session 1: Investigative Techniques Flashcards
1
Q
What is used to preserve samples in light microscopy?
A
Formalin
2
Q
What is used to embed samples for light microscopy?
A
Melted paraffin (allows sample to be sliced thinly as it sets hard once cooled)
3
Q
What does haematoxylin stain?
A
Stains the nucleus blue.
4
Q
What does eosin stain?
A
Cytoplasm and ECM pink
5
Q
TRUE OR FALSE: high frequency waves have a longer wave length than low frequency waves.
A
False
6
Q
What does TEM stand for?
A
Transmission electron microscopy
7
Q
What is a disadvantage of TEM?
A
Uses an electron beam generated in a vacuum therefore cannot be used for living samples.
8
Q
What is a sample fixed with during TEM?
A
Glutaraldehyde
9
Q
What is a sample embed in during TEM?
A
Epoxy resin
