Serology & Molecular Flashcards
Antibody dependent cell-mediated cytotoxicity (ADCC)
Cr51 labeled cells - cell death measures to determine red cell survival (extra cellular lysis)
Heterogenous mix of antibodies derived from (natural) immune response of multiple B-cells, each recognizing a different epitope on same antigen
Polyclonal
Antibodies from a single b-cell parent clone. Only recognize a single epitope per antigen
Monoclonal
(Less likely to cross react with other proteins)
Distinguish anti-D from LW(a) by DTT treatment
LW is intracellular adhesion molecule: ICAM-4
LW(a) is DTT sensitive; weak on adult D- red cells
Difference between DNA and RNA
Thymine (DNA), Uracil (RNA)
RNA is single stranded
DNA —>transcription—>RNA—>translation—>protein
Central dogma of biology
Antigens Strong on cord cells
Little i, LWa and LWb
Negative on cord cells
Lea, Leb, Sda, Ch, Rg, AnWj
Potentiator that reduces repulsive charge between cells
Albumin
Potentiator that reduces Zeta potential and accelerates antibody/antigen binding
LISS
Potentiator that accelerates binding by steric exclusion of water molecules
PEG
Chemical that cleaves negative charged sialic acid molecules from polysaccharide chains to reduce surface charge
Enzymes
rBGA (recombinant blood group antigens)
Help to identify anti-DOa by inhibition
Rh17 (Hro) produced by
D deletion (D- -) and Rh null
Rh 29 expressed on all cells except
Rh null
