Blood Products & Collection Flashcards
Donor temperature and blood pressure requirement?
=< 37.5C (99.5F)
90-180/
50-100 mmHg
Minimum Hgb/Hct for donors (female, male, autologous)
12.5/38 female
13.0/39 male
11/33 autologous
Most common donation adverse reaction
- # 1 Hematoma (9-16%)
- # 2 Vasovagal/pre-faint (2-5%)
- Others: <0.5%
Vaccines with 2 week deferral
MM Y Ty Po
Measles, mumps, yellow fever, typhoid, polio
Name 3 vaccines with 4 week deferral
- Chickenpox/shingles (Varicella zoster),
- German Measles
Hepatitis EXPOSURE deferral
12 months
Acute or chronic HepB, symptomatic HepC
3 permanent deferrals
- Confirmed positive HBsAg
- Family history CJD
- Allogeneic dura mater transplant
Deferral for Hepatitis B prophylactic vaccine
NO deferral!
Deferral for vCJD
Indefinite:
Travel to UK, France, Ireland 1980-1996, 1980-2001
Transfusion in UK, France, Ireland 1980-present
Length of deferral for donation of whole blood for Platelet (temporary drug deferral)
Aspirin- 2 days
Warfarin, heparin - 7 days
Maximum whole blood collection volume (ml/kg)
10.5 mL/kg
450+/-45ml or
500 +/- 50mL
Levels of 2,3 DPG in RbcS declines rapidly in storage, but is quickly replenished after transfusion in adults?
T or F
True
Takes longer for infants to replenish, thus fresh blood (5-7days) is requested
Successful Leokoreduction (residual wbc)
< 5.0x 10^6 and pH >6.2
Leukoreduced RBCS yield of RBCs
85% of original RbcS recovered in >=95% units tested
Acceptable leukoreduced (single) Platelet yield whole blood derived
5.5x10^10 platelets in 75% of units, <8.3x10^5 wbcs in 95%
Apheresis platelet yield (minimum)
3.0x10^11 platelets
Granulocyte minimum yield
1.0x10^10 in 75% of units
How much fibrinogen and factor 8 is contained in Cryoprecipitate
150mg fibrinogen
80IU coagulation factor 8 per unit
Low yield platelet - Minimum platelet count (pheresis). ARC Variance
2.5 - 2.9x10^11
(must be labeled with approximate yield)
To make 100ml of 2.5% saline from 12% saline:
V1 x C1 = V2 x C2
12x = 100*2.5
12x = 250
250/12 =20.83 ml
(21 ml of 12%)
Dilute to 100 ml (add 79ml DI-H20)
Platelet CCI (corrected count increment)
(POST - PRE) * BSA / plts transfused
Amount of H on cells (descending order)
O > A2 > B > A2B > A1 > A1B
% of Anti-A1 present in
-A2(sub) and
-AsubB
A2 = 1-8%
A2B = 22-35%
PAS - platelet additive solution/plasma ratio?
35% plasma, 65% PAS-C
Phosphate, Acetate, Saline -Citrate
Only FDA approved pathogen reduction technology
CERUS - INTERCEPT
Inactivates: bacteria, viruses and lymphocytes
Platelet destruction disorders: do NOT transfuse playlets
ITP, TTP, HIT (heparin induced)
Platelet dosage
5k-10k per single/random platelet
50k-100k per pheresis
Product dosing ???
5-10ml/kg. - platelets
10-20 ml/kg - plasma
Chaga’s testing (T. Cruzi)
1x per donor
Babesia testing
Required only in endemic states (14)
Minimum weight to donate
110 lbs. 50 kg
Cryoprecipitate expiration (thawed)
6 hrs after thaw (single)
4 hours after pooling
Keep at Room temp!
Irradiation parameters and indication
25-50Gy max at center
15Gy minimum
Reduce TA-GVHD by inactivating lymphocytes
2 Methods for NAT testing?
- DNA - HBV
- RNA - HIV, HCV, WNV, Babesia
PCR - amplifies DNA
TMA - amplifies RNA (no thermal cycler)
DOUBLE red donor criteria?
112 days between donations, maximum 3x per year
Male: age 17, 5’1”, 130lbs
Female: age 19, 5’5”, 150 lbs
Required Infectious disease testing
HBV surface antigen
HBcore
HCV - EIA
HCV - PCR
HIV 1/2 - eia
HIV 1/2 - PCR
HTLV 1/2 - eia
???
