Blood Products & Collection

Question 1

Donor temperature and blood pressure requirement?

Answer 1

=< 37.5C (99.5F)
90-180/
50-100 mmHg

Question 2

Minimum Hgb/Hct for donors (female, male, autologous)

Answer 2

12.5/38 female
13.0/39 male

11/33 autologous

Question 3

Most common donation adverse reaction

Answer 3

• # 1 Hematoma (9-16%)
• # 2 Vasovagal/pre-faint (2-5%)
• Others: <0.5%

Question 4

Vaccines with 2 week deferral

Answer 4

MM Y Ty Po

Measles, mumps, yellow fever, typhoid, polio

Question 5

Name 3 vaccines with 4 week deferral

Answer 5

• Chickenpox/shingles (Varicella zoster),
• German Measles

Question 6

Hepatitis EXPOSURE deferral

Answer 6

12 months

Acute or chronic HepB, symptomatic HepC

Question 7

3 permanent deferrals

Answer 7

1. Confirmed positive HBsAg
2. Family history CJD
3. Allogeneic dura mater transplant

Question 8

Deferral for Hepatitis B prophylactic vaccine

Answer 8

NO deferral!

Question 9

Deferral for vCJD

Answer 9

Indefinite:
Travel to UK, France, Ireland 1980-1996, 1980-2001

Transfusion in UK, France, Ireland 1980-present

Question 10

Length of deferral for donation of whole blood for Platelet (temporary drug deferral)

Answer 10

Aspirin- 2 days
Warfarin, heparin - 7 days

Question 11

Maximum whole blood collection volume (ml/kg)

Answer 11

10.5 mL/kg

450+/-45ml or
500 +/- 50mL

Question 12

Levels of 2,3 DPG in RbcS declines rapidly in storage, but is quickly replenished after transfusion in adults?
T or F

Answer 12

True

Takes longer for infants to replenish, thus fresh blood (5-7days) is requested

Question 13

Successful Leokoreduction (residual wbc)

Answer 13

< 5.0x 10^6 and pH >6.2

Question 14

Leukoreduced RBCS yield of RBCs

Answer 14

85% of original RbcS recovered in >=95% units tested

Question 15

Acceptable leukoreduced (single) Platelet yield whole blood derived

Answer 15

5.5x10^10 platelets in 75% of units, <8.3x10^5 wbcs in 95%

Question 16

Apheresis platelet yield (minimum)

Answer 16

3.0x10^11 platelets

Question 17

Granulocyte minimum yield

Answer 17

1.0x10^10 in 75% of units

Question 18

How much fibrinogen and factor 8 is contained in Cryoprecipitate

Answer 18

150mg fibrinogen
80IU coagulation factor 8 per unit

Question 19

Low yield platelet - Minimum platelet count (pheresis). ARC Variance

Answer 19

2.5 - 2.9x10^11
(must be labeled with approximate yield)

Question 20

To make 100ml of 2.5% saline from 12% saline:

V1 x C1 = V2 x C2

Answer 20

12x = 100*2.5
12x = 250

250/12 =20.83 ml
(21 ml of 12%)

Dilute to 100 ml (add 79ml DI-H20)

Question 21

Platelet CCI (corrected count increment)

Answer 21

(POST - PRE) * BSA / plts transfused

Question 22

Amount of H on cells (descending order)

Answer 22

O > A2 > B > A2B > A1 > A1B

Question 23

% of Anti-A1 present in
-A2(sub) and
-AsubB

Answer 23

A2 = 1-8%

A2B = 22-35%

Question 24

PAS - platelet additive solution/plasma ratio?

Answer 24

35% plasma, 65% PAS-C

Phosphate, Acetate, Saline -Citrate

Question 25

Only FDA approved pathogen reduction technology

Answer 25

CERUS - INTERCEPT

Inactivates: bacteria, viruses and lymphocytes

Question 26

Platelet destruction disorders: do NOT transfuse playlets

Answer 26

ITP, TTP, HIT (heparin induced)

Question 27

Platelet dosage

Answer 27

5k-10k per single/random platelet

50k-100k per pheresis

Question 28

Product dosing ???

Answer 28

5-10ml/kg. - platelets
10-20 ml/kg - plasma

Question 29

Chaga’s testing (T. Cruzi)

Answer 29

1x per donor

Question 30

Babesia testing

Answer 30

Required only in endemic states (14)

Question 31

Minimum weight to donate

Answer 31

110 lbs. 50 kg

Question 32

Cryoprecipitate expiration (thawed)

Answer 32

6 hrs after thaw (single)
4 hours after pooling

Keep at Room temp!

Question 33

Irradiation parameters and indication

Answer 33

25-50Gy max at center
15Gy minimum

Reduce TA-GVHD by inactivating lymphocytes

Question 34

2 Methods for NAT testing?

• DNA - HBV
• RNA - HIV, HCV, WNV, Babesia

Answer 34

PCR - amplifies DNA

TMA - amplifies RNA (no thermal cycler)

Question 35

DOUBLE red donor criteria?

112 days between donations, maximum 3x per year

Answer 35

Male: age 17, 5’1”, 130lbs
Female: age 19, 5’5”, 150 lbs

Question 36

Required Infectious disease testing

Answer 36

HBV surface antigen
HBcore

HCV - EIA
HCV - PCR

HIV 1/2 - eia
HIV 1/2 - PCR

HTLV 1/2 - eia

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