Serology

Question 1

Serology

Answer 1

Study of fluid components in the blood, especially antibodies

Question 2

Serum

Answer 2

Liquid portion of blood minus coagulation factors
Most frequently encountered specimen in immunologic testing
Separated via centrifuge

Question 3

Blood Specimen Preparation and Measuring

Answer 3

fresh serum that has not been heated
complement may need to be inactivated by Heating sample to 56°C for 30 minutes

Question 4

How to store serum if testing is delayed?

Answer 4

Between 2°C and 8°C for up to 72 hours
Frozen at –20°C or below

Question 5

What is the material that is diluted?

Answer 5

Solute

Question 6

What is the medium that makes up the rest of a solution?

Answer 6

Diluent

Question 7

How is relationship between the solute and diluent is expressed as?

Answer 7

Ratios

Question 8

Compound Dilutions

Answer 8

Are made when large dilutions are needed
Require several steps of making several smaller dilutions (serial dilutions)

Question 9

Titer

Answer 9

The last tube in which a positive reaction is visible

Question 10

Sensitivity

Answer 10

Indicates how small an amount can be measured and still produce a positive test result

Question 11

Sensitivity formula

Answer 11

%= true + / True (+) + False (-) * 100

Question 12

Specificity

Answer 12

Proportion of people who do not have a disease or condition who have a negative test

Question 13

Specificity formula

Answer 13

%= true (-)/ True (-) + False (+)

Question 14

Positive predictive value

Answer 14

The probability that a person with a positive screening test actually has the disease
%= True (+)/ True (+) + False (+)

Question 15

Negative predictive value

Answer 15

Probability that a person with a negative screening test does not have the disease
%= True (-)/ True (-) + False (-)