Serological testing Monospot test. Flashcards

1
Q

____________is used to detect infectious mononucleosis which is a self-limiting
disease caused by the Epstein-Barr virus.

A

Monospot test

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2
Q

mononucleosis It is typically seen in

A

young adults and children less than 5 years of age

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3
Q

What issue
Clinically, this disease presents as fever, malaise, lethargy, sore throat with exudates, enlarged lymph nodes on the neck, mild hepatitis, enlarged spleen, and occasional blotchy skin rash.

A

infectious mononucleosis

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4
Q

Infectious mononucleosis causes the accumulation of increased numbers and abnormal forms of lymphocytes and monocytes in the lymph nodes, thus causing formation of _______ antibody.

A

“heterophile”

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5
Q

________ is an antibody that is structurally similar to the antibody that is specifically produced as a response to antigen stimulation

A

heterophile antibody

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6
Q

True/false
Monospot test
Collect venous blood in tubes with or without anticoagulant

A

True

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7
Q

Prior to Testing Monospot test
Test the specimen within ______ of blood collection if stored between ____ degrees Celsius.

A

24 hours
2-8 degrees Celsius

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8
Q

True/False
Do not use hemolyzed or contaminated samples

A

True

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9
Q

Testing Procedure
Allow the Latex Reagent and controls to reach room temperature at what range?

A

20 to 30 degrees C

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10
Q

Testing Procedure
Gently shake the Latex Reagent vial to disperse and suspend the latex particles in the buffer solution. Vigorous shaking should be _______

A

avoided

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11
Q

True/False
Testing Procedure
Use the dispenser to transfer the specimen to the card, dispense 3 drops

A

FALSE
1 drop

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12
Q

Testing Procedure
Add ____ drop(s) of Latex Reagent next to the drop of sample

A

one

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13
Q

Testing Procedure
Gently rotate the slide for ________ manually or on a rotatory shaker set at _____ rpm.

A

3 minutes
60-100 RPM

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14
Q

Testing Procedure
Look for the presence or absence of ______ after rotating

A

agglutination

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15
Q

Testing Procedure
Report results as either

A

positive or negative

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16
Q

POS/NEG
Monospot Test Results
-Shows agglutination.

A

Positive result

17
Q

POS/NEG
Monospot Test Results
-Demonstrates a smooth, homogenous solution. No Agglutination

A

Negative result

18
Q

What ensures the accuracy and reliability of test results

A

Quality control

19
Q

Quality control Divided into two sections includes….

A

QC equipment: Calibrate to 100 rpm
CQ Reagents: positive and negative controls

20
Q

True/FAlse
Control reagents are treated as patients and run every time an unknown sample is tested.

A

True