Seperation/Isolation Techniques Flashcards
1
Q
What does a RT-PCR do?
A
measures mRNA of a protein
2
Q
What does a Southern blot do?
A
analyzes genomic DNA
3
Q
What does a Western Blot do?
A
Measures translational levels of a protein
4
Q
What does a quantitative PCR do?
A
measures DNA
5
Q
What does a Native page do?
A
separates molecules based on electrophoretic mobility
-relies on length, conformation, and charge
6
Q
What does Gel Filtration chromotography do?
A
separates proteins based off size
7
Q
What does Ion Exchange chromatography do?
A
seperates molecules based of charge
8
Q
What does isoelectric focusing do?
A
Separates molecules based on their isoelectric point
