Sensor Quiz 2 Flashcards
What is JCAD
Microbalance Based Detector
What does JCAD detect
selective for specific functional group
Interaction between CwA and polymer surface in JCAD
non-covalent
based on solubility
What type of substance does JCAD test for
Vapours and liquids
What does FTIR Spectroscopy detect
measure the amount fo light absorbed at a specific wavelngth
what does FTIR LOOK FOR
characteristics of chemical groups
True or false: The intensity of FTIR absorption is proportional to the concentration of the targeted chemical
True
Sensitivity, LOD and detecition of FTIR
High sensitivity, low LOD and fast detection of vapours
True or false: FTIR is destructive
FALSE
What kind of susbtance does THREATID device FTIR detect
Solid, liquid and gas mixture analysis
First defender device
warmup less and one second
takes 15 seconds
non-destructive
correctly identifies 78/88 CWAs
Failures in detection are below 10%
Gas phase detection of CWA with portable raman
~500 cm = bending of phosphate
~700 cm = stretching of PC
~800,830 cm = bending of phosphate 2
what is SERS spectrum
2.5ppmv
What is the intesnsity value peak at of DMMP at exposure time 2.5ppmv
~700cm
SERS Integrated Centrifugal microfluidic platform for detection facts
Concentration dependent intensity of charactereistic GA at 2100cm
Concentration dependent intensity of vx at 900cm
LOD is ~4.00ppm
what is bioterrorism
involves deliberate release of bioweapons to cause death or disease
What is Bioterrorism interpol
the spread of infectious or toxic biological agents can happen without warning
What is biotracker
consists of an itelligence repository and visualization interface which member countries can feed and benefit from
three biological agents for bioterrorism
anthrax - bacterial toxin
botulin - bacterial toxin
Ricin - plant toxin
Methods of dissemination of toxins
air, food, water, soil
Lab based methods of biological agents
Immunoassays
PCR assays
Animal assays
On-site testing methods of bioogical agents
Colorimetric detection
Fluorescent detection
electro chemical detection
What are device requirements
attract non-covalently
How to find recognition probe on ricin
protein-protein
Protein-nucleic acids
antibodies-protein
What is ricin sensor recognition probe
nucleic acid
What is a bulge
Mad eof C nuclear base
Where does probe attach to surface
Graphene surface
How is the FAM molecule attached
covalently to the 5’ end
Relationship of detection and fluorescence
increase in fluorescent dye, increase in signal
Where is the dye quenched
near carbon surfaces
A self-driven microfluidic chip in ricin detection paper facts
Measured fluorescence
Detection of AT and RT
Greater intensity of colour, higher the value
tested at 700nm
Bot, ETX, AT and PBS were negative,
Ricin was positive
What was the botulin toxin colorimetric antibody based test measured in
ng/mL - measures absorbance
increase of concentration = increase of absorbance
What is the most sensitive test
colorimetric
Anthrax spore detection using antibodies describe the test
sample goes on detection pad
Conjugate pad is where binding occurs
Spores bind to antibody
Travel through the test line and control line
antibody binds to lines
If anthrax is in sample it will be captured by antibody and both lines are coloured
Dipicolinic acid or DPA structure
Pridine, 2 carboxylic acids
What is a major component of anthrax spores
DPA, 15% of dry mass
Under UV lamp what colour does DPA positive
Red
What is the range DPA is measured in
micromolar
