Sediment Examination Technique/Microscopy/Cytodiagnostic Urine Testing

Question 1

Most frequently used stain in urinalysis

Answer 1

Sternheimer-Malbin Stain

Question 2

Components of Sternheimer-Malbin Stain

Answer 2

Crystal violet and Safranin O

Question 3

Commercially available of Sternheimer-Malbin Stain

Answer 3

Sedi stain, KOVA stain

Question 4

Used to confirm the presence of triglycerides, neutral fats and cholesterol

Answer 4

Lipid stains, Oil Red O, Sudan III and Polarizing Microscopy

Question 5

Identification of bacterial casts, which can be confused from granular cast (both has grainy appearance)

Answer 5

Gram Stain

Question 6

Components of Gram Stain

Answer 6

Crystal violet (10 stains), Iodine (Mordant), Alcohol (Decolorizer), Safranin (Counterstain)

Question 7

Preferred stain for urinary eosinophils

Answer 7

Hansel Stain

Question 8

(in cases of drug-induced allergic reaction producing inflammation of the renal interstitium)

Answer 8

Hansel Stain

Question 9

Components of Hansel Stain

Answer 9

Methylene Blue and Eosin Y

Question 10

Stain for iron

Answer 10

Prussian Blue Stain

Question 11

Color of Hemosiderin granules

Answer 11

Blue

Question 12

objects appear dark against light background,
most frequently used in the clinical laboratory

Answer 12

Bright field Microscopy

Question 13

aids in identification of Treponema pallidum

Answer 13

Dark-field Microscopy

Question 14

Enhances visualization of elements with low refractive index, such as hyaline casts, mixed cellular casts, mucous threads, and Trichomonas

Answer 14

Phase-Contrast Microscopy

Question 15

aids in identification of cholesterol in oval fat bodies, fatty casts and crystals

Answer 15

Polarizing Microscopy

Question 16

allows visualization of naturally fluorescent microorganisms or those stained by a fluorescent dye inducing labeled antigens and antibodies

Answer 16

Fluorescence Microscopy

Question 17

Produces a three-dimensional microscopy image and layer-by-layer imaging of a specimen

Answer 17

Interference Contrast

Question 18

Frequently performed independently of routine urinalysis for detection of malignancies of the lower urinary tract

Answer 18

Cytodiagnostic Urine Testing

Question 19

provides an additional method for detecting and monitoring renal disease

Answer 19

Papanicolaou stain