Secretions of the intestine, liver, gallbladder and pancreas Flashcards
what are the. primary secretions of the small intestine
intestinal juice (mucus/HCO3-) pancreatic juice (digestive enzymes) bile (bile salts)
what are key endocrine hormones involved
secretin
CKK
GIP
regulate bile and pancreatic secretions
what are the secretory cells of the SI
villi (absorptive enterocytes and mucus secreting goblet cells) intestinal glands (enterocytes secreting isotonic fluid, enteroendocrine cells, panted cells) in duodenum only (Brunner's lands secrete mucus and HCO3-)
Stem cell renewal of epithelial cells
Rapid turnover of epithelial cells every 3-6 days
differentiate and migrate moving to top of villi
Vulnerable to radiation and chemo
Secretions of SI
Intestinal juice – fluid containing electrolytes and water (secretory enterocytes), lysozyme (Paneth cells), mucus (goblet cells), alkaline mucus containing fluid (submucosal duodenal Brunner’s glands)
Key endocrine hormone secretion (by enteroendocrine cells) into vasculature
CCK (I cells) – stimulate pancreatic and gallbladder secretion
Secretin (S cells) 0 stimulate pancreatic and biliary bicarbonate secretion
GIP (K cells) – may inhibit acid secretion/stimulate insulin release
Exocrine/pancreatic juice
bicarbonate/digestive enzymes
Bile
bile salts for lipid emulsification (liver hepatocyte synthesis, gall bladder storage)
The pancreas’ secretions
Exocrine pancreas secretes pancreatic juice containing bicarbonate rich secretion (pH 8) and digestive enzymes essential for normal digestion and absorption
Pancreatic structure
Clusters of glandular epithelial clusters
99% exocrine acinar clusters secreting pancreatic juice (water, electrolytes, sodium bicarbonate, pro-enzymes)
1% endocrine pancreatic islets (islets of Langerhans) of 4 types secreting glucagon (a), insulin (B), somatostatin (delta), pancreatic polypeptide (F cell)
Exocrine acinar clusters
like grapes main duct to interlobular ducts with lobule with acinar acinar cell (cck, ach) enzyme and cl- rich secretion duct cell (secretin) HCO3- rich secretion
Acinar and ductular secretions
acinar enzymes NaCl fluid
ductular NaHCO3 fluid
Regulation of Acinar enzyme production
Ach released via ps vagus stimulation
CCK – trigger is chyme containing fat and protein products
Produces lower volume enzyme rich pancreatic juice
regulation of ductal bicarbonate and water
Secretin – trigger is H+ in highly acidic chyme
Produces copious HCO3- rich low enzyme pancreatic juice
Pancreatic enzymes
Proteolytic enzymes secreted in inactive form, proteins to peptides
Amylase hydrolyses starch, glycogen and other carbs (other than cellulose) to form di and trisaccharides
Lipases hydrolyse fat into fatty acids and monoglycerides
Nucleases digest RNA and DNA to nucleic acids
Trypsin inhibitor prevents activation to trypsin to prevent pancreatic digestion
Activation of proteolytic enzymes
Produced as inactive precursors called zymogens
SI brush border enterokinase enzyme cleaves hexapeptide to form active trypsin from trypsinogen
Trypsin cleaves and activates other proteolytic enzymes
Process prevents pancreatic autodigestion (+ trypsin inhibitor)
Duct secretion of sodium bicarbonate
Secretin stimulates high volume HCO3- rich pancreatic juce
HCO3- secretion out of cell into duct lumen via Cl/HCO3- exchange at apical membrane
Cl- recycled out of cell via CF transmembrane conductance regulator (CFTR) a CL- channel under secretin stimulation via cAMP
Na+ secreted transcellularly into duct lumen following HCO3- secretion down electrochemical gradient, water follows osmosis
Ionic composition depends on secretory rate
Unstimulated – low secretion rate, electrolyte content similar to plasma
Stimulated – high secretion rate, rise in HCO3- from ductal cells inversely related to reduced concentration of Cl- in pancreatic juice
Dysfunction in ductal CFTR Cl- channel
CF patients lack functioning channel in luminal membrane leads to defective ductal secretion
Duct blocked with precipitated enzymes and mucus, pancreas undergoes fibrosis
Blocked ducts impair secretion of pancreatic enzymes for digestion, malabsorption
Treated by taking oral pancreatic enzyme supplements with each meal
Dysfunction in enzyme activation process
Pancreatitis – inflammatory disease when pancreatic enzymes are activated in pancreas (and surrounding tissues) resulting in autodigestion of tissues
Most common cause is gallstone and alcohol abuse where obstruction of duct occurs
Role of bile in digestion
Digestion and absorption of fats Bile salts (amphipathic) emulsify fats for digestion by pancreatic lipase, solubilise fat digestion products into micelles for absorption across mucosa
role of bile in elimination of waste products
Bile pigment bilirubin from haem in RBC degradation (breakdown product stercobilin gives faecal brown colour)
Cholesterol
Drugs
Synthesis and secretion of bile in liver
Bile constantly secreted by hepatocytes lining sinusoidal blood vessels in liver acinus
Hepatocytes are key functional cell of liver, 80% mass
Bile drains into blind ended canaliculi and into bile duct for storage in gallbladder or direct drainage into duodenum
how does bile reach the gall bladder
Gallbladder via R and L hepatic ducts of liver, to cystic duct, bile duct and sphincter
Liver duct epithelial cells add water Na HCO3- to increase bile volume in response to secretin
