Searhorse Flashcards
1
Q
What does oligomycin do
A
Oligomycin inhibits ATP synthase (complex V), This decrease in OCR is linked to cellular ATP production.
2
Q
What id FCCP
A
Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone
(FCCP) is an uncoupling agent that collapses the proton
gradient and disrupts the mitochondrial membrane potential. It
is the 2nd injection following Oligomycin. As a result, electron
flow through the ETC is uninhibited, and oxygen consumption
by complex IV reaches the maximum
3
Q
What dose Rotenone and antimycin A do
A
Rotenone, a complex I inhibitor, and antimycin A, a complex III inhibitor this combination shuts down mitochondrial respiration and enables the calculation of nonmitochondrial respiration driven by processes outside the mitochondria..
