Ogg1 gene

Question 1

Where is the human OGG1 gene located?

Answer 1

On the short arm of chromosome 3 at position 25.3

Question 2

What is the length of the human OGG1 gene?

Answer 2

7,421 base pairs

Question 3

What is unique about the promoter region of the OGG1 gene?

Answer 3

It lacks a TATA or CAAT sequence and contains two CpG islands

Question 4

Which other gene overlaps with the 3’ end of the OGG1 gene?

Answer 4

Calmodulin kinase I (CaMK1)

Question 5

What are the conserved motifs found in the OGG1 gene?

Answer 5

Helix-hairpin-helix structural element, glycine/proline-rich loop, GDP motif

Question 6

What amino acids within the GDP motif are important for OGG1 activity?

Answer 6

Lys249 and Asp268

Question 7

What is the role of the MTS and NLS in OGG1?

Answer 7

MTS localizes OGG1 to mitochondria; NLS in OGG1-1a targets it to the nucleus

Question 8

How many exons does the human OGG1 gene comprise?

Answer 8

Eight exons

Question 9

How many isoforms of human OGG1 are documented?

Answer 9

Eight isoforms

Question 10

What distinguishes type 1 and type 2 OGG1 isoforms?

Answer 10

Type 1 ends with exon 7; type 2 ends with exon 8

Question 11

Which OGG1 isoforms are commonly expressed in most cell types?

Answer 11

OGG1-1a (α-hOGG1) and OGG1-2a (β-hOGG1)

Question 12

What is the homolog of the human OGG1 gene in mice?

Answer 12

Mouse Ogg1 gene

Question 13

What percentage of nucleotide sequence identity does mouse Ogg1 have compared to human OGG1?

Answer 13

81% identity

Question 14

How long is the mouse Ogg1 gene?

Answer 14

6 kb long

Question 15

How many exons does the mouse Ogg1 gene have?

Answer 15

Seven exons

Question 16

Do mouse OGG1 proteins have isoforms?

Answer 16

No, there are no isoforms for mouse OGG1

Question 17

What tissues show higher Ogg1 expression levels?

Answer 17

Nervous system and eye tissues

Question 18

Which brain regions exhibit high Ogg1 activity?

Answer 18

Hippocampus, caudate/putamen, midbrain

Question 19

What is the mitochondrial to nuclear incision activity ratio in the brain?

Answer 19

0.38

Question 20

At what developmental stage has Ogg1 mRNA been detected?

Answer 20

Blastocyst stage

Question 21

How does OGG1 activity in fetal tissue compare to adult tissues?

Answer 21

Higher in fetal tissue

Question 22

What is the trend of OGG1 activity with age?

Answer 22

Age-dependent decline

Question 23

What circadian pattern does OGG1 expression follow?

Answer 23

Peaks at 8 AM, decreases by 40% by 8 PM

Question 24

Which proteins are involved in the transcriptional regulation of Ogg1?

Answer 24

SP1 and NRF2/C-ETS1