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Immuno-sero > Screening for Phagocytic Engulfment > Flashcards

Screening for Phagocytic Engulfment Flashcards

1
Q

Steps of Phagocytosis

A
  1. Chemotaxis
  2. Adherence
  3. Engulfment
  4. Phagosome formation
  5. Fusion
  6. Digestion and destruction
  7. Exocytosis
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2
Q

The movement of an organism or entity in response to a chemical stimulus

A

Chemotaxis

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3
Q

Types Of phagocytes

A

A.Monocyte Macrophage System

B.Neutrophil

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4
Q

A useful test of impaired neutrophilic function

A

Phagocytic Engulfment

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5
Q

Positive result of screening for phagocytic engulfment

A

demonstration of bacterial engulfment

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6
Q

Negative result of screening for phagocytic engulfment

A

No bacterial engulfment

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7
Q

Lens objective use in screening for phagocytic engulfment

A

Focus first using high power objective then proceed to oil immersion objective

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8
Q

Stain use for phagocytic engulfment

A

Wright stain

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9
Q

How many drops of buffy coat is added to the test tube

A

4-8 drops

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10
Q

How many drops of bacterial broth culture

A

2-3 drops

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11
Q

Indicates correct buffer to stain ration

A

greenish metallic sheen

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12
Q

Water bath is set at what temperature in screening for phagocytic engulfment

A

37 degrees celsius

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13
Q

Predominant phagocytic cell

A

Neutrophil

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14
Q

what does it mean if LYMPHOCYTES are the only WBC seen in screening for phagocytic engulfment

A

bacterial suspension is too heavy and phagocytic cells destroy themselves in attempt to engulf the bacteria present

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15
Q

Possible cause of false negative results

A
  • Spx is not fresh
  • use of coagulase positive S. aureus and encapsulated bacteria
  • neutrophil disorders
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16
Q

Bacteria must be in what state for the test to be positive

A

INTRACELLULAR

17
Q

T/F

Presence of engulfed bacteria in the screening test does NOT demonstrate that bacteria has been destroyed

A

TRUE

18
Q

How long should it take to incubate in water bath?

A

30 mins

19
Q

blood smear is watery

A

due to addition of bacterial broth culture making it difficult to achieve FEATHERED EDGE bloodsmear

20
Q

specimen for screening for phagocytic engulfment

A

Fresh whole blood collected in EDTA

Immuno-sero (4 decks)

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