FLOCCULATION TEST

Question 1

NON-TREPONEMAL FLOCCULATION TEST to detect & QUANTIFY reagin antibodies associated with Syphilis

Answer 1

RAPID PLASMA REAGIN

Question 2

Specimen for Rapid plasma reagin flocculation test

Answer 2

Fresh, non-hemolyzed Unheated Serum

Question 3

ANTIGEN use in RPR

Answer 3

cardiolipin suspension( modification of VDRL antigen) containing microparticulate charcoal

Question 4

What is the use of the subtance found in the RPR antigen?

Answer 4

microparticulate charcoal = enhance the visual difference between a positive and negative result

Question 5

Diluent for titration in RPR

Answer 5

0.9 % Normal saline solution

Question 6

capable of dispensing 50 microliters

Answer 6

disposable dispensers for serum/ plasma

Question 7

specimen-antigen supension remains HOMOGENOUS

Answer 7

non-reactive specimen

Question 8

Suspension must be totally HOMOGENOUS before use

Answer 8

True

Question 9

stabilized liquid control, reactive with RPR antigen

Answer 9

RPR control serum positive (red cap)

Question 10

stabilized liquid control,nonreactive with RPR antigen

Answer 10

RPR control serum negative (green cap)

Question 11

cardiolipin suspension containing microparticulate charcoal

Answer 11

RPR antigen suspension (white cap)

Question 12

Hold dispenser bottle VERTICALLY and do not restir during Qualitative test

Answer 12

True

Question 13

How many minutes do you tilt RPR reaction card ?

Answer 13

8 minutes

Question 14

what light do you use to read results?

Answer 14

bright artificial light

Question 15

Large aggregates in the center or the PERIPHERY of the test rings.

Answer 15

Reactive result

Question 16

Samples with reactive test Results should be retested in the SEMI-QUANTITATIVE test

Answer 16

TRUE

Question 17

presence of small aggregates around the edge of the test ring

Answer 17

Very weak reactive

Question 18

RPR result with smooth, even appearance with no aggregates

Answer 18

Negative

Question 19

Reciprocal of the last dilution that contains macroscopic aggregates

Answer 19

titer

Question 20

Diseases that may cause FALSE POSITIVE RESULT in RPR tests

Answer 20

SIM - LVV

• LEPROSY
• SYSTEMIC LUPUS ERYTHEMATOSUS
• INFECTIOUS MONONUCLEOSIS
• MALARIA
• VACCINIA
• VIRAL PNEUMONIA

Question 21

Reactive test samples should undergo what test?

Answer 21

serological test ( TPHA )

Question 22

Final diagnosis of RPR , should be based on what?

Answer 22

correlation of test results with other clinical findings

Question 23

Contaminated sera in RPR

Answer 23

False positive result

Question 24

Longer reaction time

Answer 24

false positive result

Question 25

what kind of saline do you use in semi-quantitative method?

Answer 25

isotonic saline

Question 26

should be performed once in 3 months

Answer 26

External Quality Control

Question 27

Positive & negative internal kits controls alongside px. samples

Answer 27

Internal Quality control

Question 28

confirmation testing for Reactive RPR test

Answer 28

specific treponemal tests: Treponema pallidum hemagglutination

Question 29

sera-containing anti reagin antibodies

Answer 29

flocculation of antigen-charcoal particles is observed

Question 30

mechanical rotator at what rpm

Answer 30

180 rpm circumstriving of 2 cm in diameter on horizontal plane

Question 31

large and medium black floccules againts white background

Answer 31

Reactive

Question 32

no floccules, even grey background

Answer 32

Non-reactive

Question 33

weakly reactive

Answer 33

small black floccules against white background

Question 34

indicates absence of anti-lipoidal antibodies

Answer 34

non-reactive result

Question 35

presence of reagin or antilipoidal antibodies

Answer 35

reactive results