FLOCCULATION TEST Flashcards
NON-TREPONEMAL FLOCCULATION TEST to detect & QUANTIFY reagin antibodies associated with Syphilis
RAPID PLASMA REAGIN
Specimen for Rapid plasma reagin flocculation test
Fresh, non-hemolyzed Unheated Serum
ANTIGEN use in RPR
cardiolipin suspension( modification of VDRL antigen) containing microparticulate charcoal
What is the use of the subtance found in the RPR antigen?
microparticulate charcoal = enhance the visual difference between a positive and negative result
Diluent for titration in RPR
0.9 % Normal saline solution
capable of dispensing 50 microliters
disposable dispensers for serum/ plasma
specimen-antigen supension remains HOMOGENOUS
non-reactive specimen
Suspension must be totally HOMOGENOUS before use
True
stabilized liquid control, reactive with RPR antigen
RPR control serum positive (red cap)
stabilized liquid control,nonreactive with RPR antigen
RPR control serum negative (green cap)
cardiolipin suspension containing microparticulate charcoal
RPR antigen suspension (white cap)
Hold dispenser bottle VERTICALLY and do not restir during Qualitative test
True
How many minutes do you tilt RPR reaction card ?
8 minutes
what light do you use to read results?
bright artificial light
Large aggregates in the center or the PERIPHERY of the test rings.
Reactive result
Samples with reactive test Results should be retested in the SEMI-QUANTITATIVE test
TRUE
presence of small aggregates around the edge of the test ring
Very weak reactive
RPR result with smooth, even appearance with no aggregates
Negative
Reciprocal of the last dilution that contains macroscopic aggregates
titer
Diseases that may cause FALSE POSITIVE RESULT in RPR tests
SIM - LVV
- LEPROSY
- SYSTEMIC LUPUS ERYTHEMATOSUS
- INFECTIOUS MONONUCLEOSIS
- MALARIA
- VACCINIA
- VIRAL PNEUMONIA
Reactive test samples should undergo what test?
serological test ( TPHA )
Final diagnosis of RPR , should be based on what?
correlation of test results with other clinical findings
Contaminated sera in RPR
False positive result
Longer reaction time
false positive result
what kind of saline do you use in semi-quantitative method?
isotonic saline
should be performed once in 3 months
External Quality Control
Positive & negative internal kits controls alongside px. samples
Internal Quality control
confirmation testing for Reactive RPR test
specific treponemal tests: Treponema pallidum hemagglutination
sera-containing anti reagin antibodies
flocculation of antigen-charcoal particles is observed
mechanical rotator at what rpm
180 rpm circumstriving of 2 cm in diameter on horizontal plane
large and medium black floccules againts white background
Reactive
no floccules, even grey background
Non-reactive
weakly reactive
small black floccules against white background
indicates absence of anti-lipoidal antibodies
non-reactive result
presence of reagin or antilipoidal antibodies
reactive results
