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Dengue Flashcards

1
Q

What do you detect in serologic tests for dengue

A

IgG ,IgM, NS1 antigen (non-structural protein 1)

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2
Q

Severe form of Dengue Fever

A

Dengue Hemorrhagic Fever

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3
Q

Single positive stranded RNA virus

A

Dengue Virus

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4
Q

What mosquito transmits dengue?

A 
Aedes aegypti (mainly)
Aedes albopictus (ocassionally)
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5
Q

Where is A. aegypti usually located?

A

urban, suburban. manmade containers like tires, flower pots, household water

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6
Q

4 serotypes

A

DENV 1,DENV-2, DENV-3,DENV4

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7
Q

recovery from infection is believed to provide LIFELONG IMMUNITY against serotype

A

TRUE

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8
Q

CROSS-IMMUNITY to other serotypes after recovery is only partial and temporary

A

TRUE

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9
Q

transported to america & eurpose by asian products like bamboos & trees

A

A. albopictus

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10
Q

Secreted into blood during dengue infection

A

NS1

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11
Q

When is NS1 detectable ?

A
  • Acutephase *
    within 1-2 days ff infection ;9 days ff symptom onset
    *SECONDARY dengue virus infection
    1-4 days ff symptom onset
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12
Q

High titers as seen in primary infection

A

IgM

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13
Q

Classified as presumptive recent dengue virus infections

A

Positive IgM result

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14
Q

Detectable 4- 5 days after onset symptoms and detectable approx. for 12 wks

A

IgM

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15
Q

by 3 weeks ff.exposure of this type of antibody, nearly all immunocompetent individuals should have develop IgG antibodies to DENV

A

igG

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16
Q

presence of this type of antibody indicates exposure to the virus sometime in the past

A

igG

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17
Q

Principle of rapid test kits for Dengue

A

Lateral flow immunochromatographic assay

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18
Q

Dengue Shock syndrom treatment

A
  • hospitalization in an intensive care unit for close monitoring and administration of medication that raises Blood pressure into normal range
  • IV fluids
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19
Q

Increased permeability of blood vessels

A

plasma leakage

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20
Q

DHF 4 key features

A
  • Increased permeability of Blood vessels
  • Low blood platelet count
  • fever lasting 2-7 days
  • tendency toward severe hemorrhagic bledding
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21
Q

Most dangerous symptom of Dengue

A

Increased permeability of blood vessels leading to plasma leakage that may cause SHOCK

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22
Q

inability to circulate blood properly bcs there is not enough fluid left in the blood vessel is

A

SHOCK

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23
Q

shock can lead to

A
  • organ failure

* metabolic problems

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24
Q

Where and when was the first vaccine of Dengue made available

A

Mexico, December 2015

25
Q

Age bracket for Dengue vaccine

A

9-25 yrs old( 3 dose series )

26
Q

Diagnosis of early acute dengue infection

A

Dengue NS1 Ag test device

27
Q

designed for the rapid qualitative, differential detection of antibodies to dengue virus

A

IgG/IgM test device

28
Q

Conjugate pad for Dengue Ns1 Ag test device

A

mouse monoclonal anti-dengue NS1-gold colloid

29
Q

NS1 -Test line

A

mouse monoclonal anti-dengue NS1

30
Q

Control line for NS1 test device

A

goat anti-mouse IgG

31
Q

Conjugate pad for Dengue IgG/IgM test device

A

recombinant dengue virus envelope protein-gold colloid

32
Q

Test line “G” for Dengue IgG/IgM test device

A

mouse monoclonal antihuman IgG

33
Q

Test line “M” for Dengue IgG/IgM test device

A

mouse monoclonal antihuman IgM

34
Q

Control line for Dengue IgG/IgM test device

A

rabbit anti-dengue IgG

35
Q

Absence of Control Bands

36
Q

Dengue NS1 antigen positive test result

A

purple colored T band

37
Q

When specimen is added into sample well, what mechanism of action happens when spx migrates across the cassette

A

capillary action

38
Q

Specimen for dengue test

A

Fresh non hemolyzed serum obtained by venipuncture

39
Q

Interpret results of Dengue NS1 Antigen and IgG/IgM

A

15-20 minutes

40
Q

C- Band is present and absence of color in Dengue NS1 antigen

A

Negative result

41
Q

Presence of C- band and T-band in Dengue NS1 Test kit

A

POsitive result

42
Q

No C-band is developed but shows purple color in T-band

43
Q

Repeat assay using a new device

A

invalid result

44
Q

Dengue IgG / IgM negative result

A

Retest in 3-5 days if dengue is suspected

45
Q

only C -band is present in Dengue IgG/IgM test kit

46
Q

Presence of C-band, “M” band is developed in Dengue IgG/IgM test kit

A

IgM Positive (Primary dengue Infection)

47
Q

Presence of C -band, “G” band is developed in Dengue IgG/IgM test kit

A

IgG positive ( Secondary or Past Dengue infection)

48
Q

Result of M / G line is weak in Dengue IgG/IgM test kit

A

Positive result

49
Q

Presence of C-band , “G” and “M” bands in Dengue IgG/IgM test kit

A

Both IgG and IgM positive (Late primary or early secondary dengue infection)

50
Q

Most likely reasons for control line failure

A
  • Insufficient spx volume

- Incorrect procedural technique

51
Q

a negative test result cannon exclude a recent infection

52
Q

presence of detectable NS-1 antigen

A

may mean positive for early dengue infection

53
Q

detectable levels of IgM may be LOW

A

early primary infections and some secondary infections

54
Q

Some patients may nit produce detectable levels of antibody within how many days

A

first 7-10 days after infection, when symptoms persist px should be retested 3-4 days after the first specimen

55
Q

Expected to be detected 1 day after onset of fever and persist up to 9 days in both primary and secondary infection

A

NS-1 antigen

56
Q

anti-NS 1 antibodies are produced

A

detection of NS-1 antigen is inhibited

57
Q

characterized by the presence of detectable IgM 3-5 days after onset of symtomps

A

Primary dengue

58
Q

characterized by presence of specific IgG 1-2 days after onset of infection, generally accompanied by elevation of IgM

A

Secondary dengue

59
Q

Serological cross reactivity across the Flavivirus is common

A

DI- SAD-JOD-WEgtengen-YEs!

  • Dengue virus
  • St.Louis encephalitis
  • Japanese encephalitis,
  • West nile & yellow fever virus

Immuno-sero (4 decks)

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