scientific skills Flashcards
when do you use correlation coefficient?
when examining relationships between variables
Use the Pearson correlation coefficient.
Spearman’s rank correlation coefficient is the non-parametric equivalent of the Pearson correlation coefficient.
when do you use a paired sample t-test?
when examining differences between groups
the same participants are being tested more than once
two groups
when do you use a repeated measure ANOVA?
when examining differences between groups
the same participants are being tested more than once
more than 2 groups
when do you use an independent samples t-test?
when examining differences between groups
the participants are only being tested once
two groups
when do you use a one-way ANOVA
when examining differences between groups
the participants are only being tested once
more than 2 groups
advantage of Turkeys
it can be used for groups of equal and unequal sizes
assumptions of a parametric test
the data are continuous
the data are normally distributed
the variances of each group are statistically equal
rules of data for t test and ANOVA
must be performed on normally-distributed, continuous data with equal group variances
phosphorylation
addition of a phosphate group to oxygen of serine, theoine and tyrosine
myristoylation
attachment of 14-carbon fatty acid to N terminus of a protein
attachment is via alpha amino group
chain assists with membrane
restriction endonucleases
cut DNA at specific nucleotide sequences
low kd
high affinity interaction, takes less ligand to fill half the protein
EC50
concentration that gives half the maximal response
threshold
minimum concentration that produces a quantifiable response
potency
measure of concentration of drug needed to elicit a response
-use EC50
intrinsic activity
measures ability of a drug to elicit a response when it occupies a receptor
full agonist
can elicit a maximum response- has an intrinsic activity of 1
partial agonist
cannot elicit a maximum response- intrinsic activity of less than one
smaller the Kb
the higher the affinity antagonist
equation for conc of DNA
= A260 x dilution factor x 50ug/ml
stokes shift
the difference in the maxima between the excitation and emission wavelength spectra
the greater the difference, the easier it is to separate the emitted light from the excitation light
transition value
value at which 50% of light is transmitted
primary antibody
antibody that recognises the protein of interest
nernst equation
allows us to calculate the electrical potential required to balance a given concentration gradient of an ion
advantage of confocal microscopy
it reduces out of focus fluoresce
