SB1h Enzyme Activity

Question 1

Describe the effect of temperature on enzyme

activity.

Answer 1

Low temperature affect is molecules move slowly. substrate molecules take longer to fit into and react with the active site
At optimum it works the best
high temeprature causes change to active site, so can not hold substrate tightly
very high temperature, active site breaks up, and enzyme is denatured.

Question 2

Describe the effect of substrate concentration on

enzyme activity

Answer 2

Increasing substrate reaction also increases rate of reaction to a point, once all enzymes are catalyzed, any substrate increased will have no effect.

Question 3

Describe the effect of pH on enzyme activity

Answer 3

At very acidic and alkaline pH values the shape of the enzyme is altered so that it is no longer complementary to its specific substrate

Question 4

Explain what is meant by the optimum

pH/temperature of an enzyme

Answer 4

temperature/pH which works at the best.

Question 5

Calculate the rate of enzyme activity from

experimental data.

Answer 5

1/time

Question 6

Explain why temperature, substrate concentration

and pH affect enzyme activity.

Answer 6

Substrate requires different temperatures, and pH