Core Practical Using Microscopes

Question 1

Making a wet mount slide

Answer 1

1. Place a thin section of the specimen onto slide.
2. Place a drop of water in the middle of the slide or stain the specimen.
3. Gently lower cover slip onto the specimen without trapping air bubbles.
4. Soak up any excess liquid with a paper towel.
5. Switch on the light source and place your slide on the stage.
6. Use the lowest objective lens and turn the focusing wheel to move the lens close to the slide.
7. Slowly adjust the focusing wheel until you can see a clear image.
8. Increase the magnification by changing the objective lens and re-focus.