RPAs Flashcards

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1
Q

What is the food test for reducing sugars?

A

Benedict’s

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2
Q

What is the food test for starch?

A

Iodine

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3
Q

What is the food test for protein?

A

Biuret

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4
Q

What is the food test for lipids?

A

Ethanol

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5
Q

What is different about the food test for lipids?

A

Don’t filter solution

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6
Q

How much food solution is put into the test tube?

A

2cm^3

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7
Q

How do you make the food solution?

A

Take the food sample and grind with a pestle and mortar with some distilled water to create a paste
Transfer paste to beaker and add more distilled water, stir so the chemicals in the food dissolve in the water

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8
Q

For very food test apart from lipids, what do you need to do after stirring the food paste with distilled water?

A

Filter solution to remove suspended food particles

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9
Q

How many drops of iodine solution do you add?

A

A few

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10
Q

How many drops of Benedict’s solution do you add?

A

10

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11
Q

After adding Benedict’s solution, what do you need to do?

A

Place in a hot water bath for 5 minutes

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12
Q

How much biuret solution do you add?

A

2cm^3

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13
Q

How much distilled water and ethanol do you add?

A

A few drops of each

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14
Q

What do you do after adding ethanol and distilled water?

A

Gently shake

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15
Q

What is the negative test colour for starch?

A

Orange

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16
Q

What is the positive test colour for starch?

A

Blue-black

17
Q

What is the negative test colour for reducing sugars?

A

Green/ green-yellow

18
Q

What is the positive test colour for reducing sugars?

A

Brick red / orange

19
Q

What is the negative test colour for protein?

A

Blue

20
Q

What is the positive test colour for protein?

A

Lilac

21
Q

What is the negative test colour for lipids?

A

Transparent

22
Q

What is the positive test colour for lipids?

A

Cloudy emulsion