RPAs Flashcards
What is the food test for reducing sugars?
Benedict’s
What is the food test for starch?
Iodine
What is the food test for protein?
Biuret
What is the food test for lipids?
Ethanol
What is different about the food test for lipids?
Don’t filter solution
How much food solution is put into the test tube?
2cm^3
How do you make the food solution?
Take the food sample and grind with a pestle and mortar with some distilled water to create a paste
Transfer paste to beaker and add more distilled water, stir so the chemicals in the food dissolve in the water
For very food test apart from lipids, what do you need to do after stirring the food paste with distilled water?
Filter solution to remove suspended food particles
How many drops of iodine solution do you add?
A few
How many drops of Benedict’s solution do you add?
10
After adding Benedict’s solution, what do you need to do?
Place in a hot water bath for 5 minutes
How much biuret solution do you add?
2cm^3
How much distilled water and ethanol do you add?
A few drops of each
What do you do after adding ethanol and distilled water?
Gently shake
What is the negative test colour for starch?
Orange