RP1

Question 1

What are the factors that affect enzyme activity? (4)

Answer 1

• Enzyme concentration
• Substrate concentration
• Temperature
• pH

Question 2

How is a control set up in a practical measuring enzyme activity?

Answer 2

Replace the enzyme solution with distiller water or boiled enzyme solution

Question 3

How can the results of the practical measuring enzyme activity be used to find the initial rate of reaction?

Answer 3

Plot your results on a graph of ‘rate of reaction’ against ‘time’
Draw a tangent at time = 0 to find the initial rate

Question 4

How is the rate of reaction calculated from time?

Answer 4

Rate of reaction = 1/time

Question 5

What is the effect of temperature on enzyme activity?

Answer 5

Temp increases -> KE increases so more ESC form -> rate increases up to optimum temps

After optimum temps tertiary structure changes, bonds break, active site changes -> substrate no longer complementary so no more ESC form -> rate of reaction decreases.

Question 6

What is the risk associated with handling enzymes?

Answer 6

Students may have allergic reactions to enzymes, so avoid contact with skin and eyes, wear eye protection.