RNA transcription Flashcards
name the differences between RNA and DNA
different sugars ribose has one more oxygen than deoxyribose RNA has uracil DNA is double stranded DNA is more stable
is the antisense or sense strand transcribed?
antisense
is the sense strand or antisense strand identical to mRNA?
sense strand
name and describe the steps of transcription
Intiation - when RNA polymerase binds to the antisense strand
Elongation - polymerase transcribes the gene
Termination - polyermase stop transcribing
Processing - mature mRNA is formed
export - mRNA leaves the nucleus
what are the types of RNA polymerase?
RNA polymerase I, II and III
what does RNA polymerase I code for?
rRNA
what does RNA polymerase II code for?
mRNA
what does RNA polymerase III code for?
tRNA
what is the initiating methionine?
ATG
what does the RNA initially bind to?
start codon - ATG
what is the promoter region and what does it do?
TATA box upstream of the start codon
attaches RNA polymerase II to the gene
what do transcription factors do?
proteins that bind to specific DNA sequences and either recruit or block RNA polymerase binding
what regions can transcription factors therefore bind to?
enhancer regions - activator proteins bind to increase transcription rate
inhibitor regions - repressor proteins bind to inhibit transcription
what direction does RNA polymerase add bases in?
3 prime to 5 prime
where does transcription stop?
stop codon
