Rhabdovirus

Question 1

Rhabdoviruses include? whats main one?

Answer 1

Vesiculovirus (vesicular stomatitis viruses)

Lyssavirus: RABIES

Question 2

Rhabdo structure

Answer 2

• bullet shaped
• enveloped
• -ssRNA

Question 3

Replication

Answer 3

adsoprtion via attachment G protein

replicates in cytoplasm

Question 4

Phases

Answer 4

Incubation phase
Prodromal phase
neurologic phase

Question 5

Incubation phase

Answer 5

no clinical signs

Question 6

Prodromal phase

Answer 6

Virus travelling to CNS. travels within axons via retrograde fast axonal transport. replicates in motor neurons of spinal cord. travels up dorsal root ganglion..
SYMPTOMS: fever, nausea, vomiting, loss of appetite, lethargy, headache.

Question 7

Neurological phase

Answer 7

Infection of the brain neurons. hippocampus, brainstem, pontine nuclei, purkinje cells of cerebellum, travels to salivary glands, cornea, other organs.
SYMPTOMS:
hydrophobia, pharyngeal spasms, hyperactivity, anxiety, depresion, loss of coordination, paralysis, dellerium, donfusion, coma, death.

Question 8

Epidemiology

Answer 8

zoonotic, wildlife and unvaccinated pets.
saliva of rabid animals.
(minorly spread via aerosols of bat caves.)

Question 9

Diagnosis

Answer 9

neurological symptoms = too late for treatment(you dying)
antigen detection: IF or genome detection by RT-PCR
Negri bodies in affected neurons (biopsy)
ELISA for rabies antibody in serum and CSF

Question 10

Treatment

Answer 10

postexposure rabies vaccine (4 within 2 weeks).

Always fatal unless treated with postrabies immunization. One case of successful cessation of disease.

Question 11

Rabies Vaccine

Answer 11

Killed-Virus vaccine
chemical inactivation of rabies infected-tissue culture human diploid cells.
Animal workers and lab workers receive preexposure vaccination.