Review Cards - Urinalysis & Body Fluids Flashcards
Urine specimens - random - use
routine urinalysis
Urine specimens - random - collection
Any time
Urine specimens - random - not ideal?
Because urine may be dilute & contaminated
Urine specimens - first morning - use
Routine urinalysis
Urine specimens - first morning - collection
Upon waking
Urine specimens - 2-hour postprandial - use
Diabetes mellitus monitoring
Urine specimens - 2-hour postprandial - collection
2 hours after eating
Urine specimens - 2-hour postprandial - best for detecting?
glycosuria
Urine specimens - 24-hour - use
quantitative chemical tests
Urine specimens - 24-hour - collection
-discard 1st void on day 1 & note time
-collect all urine for next 24 hours, including 1st void at same time on day 2
Urine specimens - 24-hour - common source of error
improper collection
Urine specimens - clean catch - use
routine, culture
Urine specimens - clean catch - collection
cleanse external genitalia & collect midstream in sterile container
Urine specimens - catheterized - use
culture
Urine specimens - catheterized - collection
catheter inserted into urethra
Urine specimens - suprapubic aspiration - use
culture
Urine specimens - suprapubic aspiration - collection
needle inserted through abdomen into bladder
Urine volume - normal daily volume
600-2,000 mL (average 1,200-1,500 mL)
Urine volume - normal day-to-night ratio
2:1 - 3:1
Urine volume - diuresis
increased urine production
Urine volume - polyuria
Marked increase in urine flow
-adult: >2,500 mL/day
-children: 2.5-3 mL/kg/day
Urine volume - oliguria
Marked decrease in urine flow
-adult: <400 mL/day
-children: <0.5 mL/kg/hour
-infants: <1 mL/kg/hour
Urine volume - anuria
no urine production
Urine color - normal
yellow due to urochrome
Urine color - diluted urine
colorless, pale yellow
Urine color - concentrated urine
dark yellow, amber
Urine color - bilirubin
amber, orange, yellow-green; yellow foam on shaking
Urine color - urobilin
amber, orange; NO yellow foam in shaking
Urine color - homogentisic acid
normal on voiding; brown or black on standing
Urine color - melanin
brown or black on standing
Urine color - methemoglobin
brown or black
Urine color - myoglobin
red; brown on standing
Urine color - blood/hemoglobin
pink or red when fresh; brown on standing
Urine color - porphyrin
port-wine
Urine color - drugs, medications, food
green, blue, red, orange
Urine color - Pseudomonas infection
green, blue-green
Changes in unpreserved urine at room temperature >2 hours - turbidity
increased
-cause:
—multiplication of bacteria
—precipitation of amorphous crystals
Changes in unpreserved urine at room temperature >2 hours - pH
increased
-cause: conversion of urea to ammonia by bacteria
Changes in unpreserved urine at room temperature >2 hours - glucose
decreased
-cause: metabolism by bacteria
Changes in unpreserved urine at room temperature >2 hours - ketones
decreased
-cause:
—volatilization of acetone
—breakdown of acetoacetate by bacteria
Changes in unpreserved urine at room temperature >2 hours - urobilinogen
decreased
-cause: oxidation to urobilin
Changes in unpreserved urine at room temperature >2 hours - bilirubin
decreased
-cause: oxidation to biliverdin
Changes in unpreserved urine at room temperature >2 hours - WBCs, RBCs, casts
decreased
-cause: lysis in dilute or alkaline urine
Chemical urinalysis by reagent strip - pH - normal
First AM: 5-6
Random: 4.5-8
Chemical urinalysis by reagent strip - pH - principle
double indicator system
Chemical urinalysis by reagent strip - pH - significance
-acid-base balance
-management of UTI/renal calculi
Chemical urinalysis by reagent strip - pH - acid
protein/meat diet
Chemical urinalysis by reagent strip - pH - alkaline
vegetarian diet
Chemical urinalysis by reagent strip - pH - pH 9
Improperly preserved specimen
Chemical urinalysis by reagent strip - protein - normal
negative - trace
Chemical urinalysis by reagent strip - protein - principle
protein error of indicators
Chemical urinalysis by reagent strip - protein - significance
possible renal disease
Chemical urinalysis by reagent strip - protein - buffered to a pH of?
3
Chemical urinalysis by reagent strip - protein - most sensitive to?
albumin
Chemical urinalysis by reagent strip - protein - orthostatic proteinuria
-benign condition
-protein is negative in 1st AM specimen
-protein is positive after standing
Chemical urinalysis by reagent strip - glucose - normal
Negative
Chemical urinalysis by reagent strip - glucose - principle
glucose oxidase/peroxidase
Chemical urinalysis by reagent strip - glucose - significance
possible diabetes mellitus
Chemical urinalysis by reagent strip - glucose - specific for?
glucose
Chemical urinalysis by reagent strip - glucose - normal renal threshold
160-180 mg/dL
Chemical urinalysis by reagent strip - ketones - normal
negative
Chemical urinalysis by reagent strip - ketones - principle
sodium nitroprusside reaction
Chemical urinalysis by reagent strip - ketones - significance
Increased fat metabolism:
-uncontrolled diabetes mellitus
-vomiting
-starvation
-low carb diet
-strenuous exercise
Chemical urinalysis by reagent strip - ketones - most sensitive to?
Acetoacetic acid
Chemical urinalysis by reagent strip - ketones - less sensitive to?
acetone
Chemical urinalysis by reagent strip - ketones - doesn’t react with?
beta-hydroxybutyric acid
Chemical urinalysis by reagent strip - blood - normal
negative
Chemical urinalysis by reagent strip - blood - principle
pseudoperoxidase activity of hemoglobin
Chemical urinalysis by reagent strip - blood - significance
-hematuria
-hemoglobinuria
-myoglobinuria
Chemical urinalysis by reagent strip - blood - uniform color vs. speckled
-uniform color: HGB or myoglobin
-speckled: RBCs
Chemical urinalysis by reagent strip - blood - bilirubin - normal
negative
Chemical urinalysis by reagent strip - blood - bilirubin - principle
Diazo reaction
Chemical urinalysis by reagent strip - blood - bilirubin - significance
-liver disease
-biliary obstruction
Chemical urinalysis by reagent strip - blood - bilirubin - what type excreted in urine?
conjugated bilirubin
Chemical urinalysis by reagent strip - blood - urobilinogen - normal
1 Ehrlich Unit or 1 mg/dL
Chemical urinalysis by reagent strip - blood - urobilinogen - principle
Ehrlich aldehyde reaction or diazo reaction
Chemical urinalysis by reagent strip - blood - urobilinogen - significance
-liver disease
-hemolytic disorders
Chemical urinalysis by reagent strip - blood - urobilinogen - reagent strips DO NOT detect?
they do not detect ABSENCE of urobilinogen, only increased
Chemical urinalysis by reagent strip - blood - nitrite - normal
negative
Chemical urinalysis by reagent strip - blood - nitrite - principle
Greiss reaction
Chemical urinalysis by reagent strip - blood - nitrite - significance
UTI
Chemical urinalysis by reagent strip - blood - nitrite - bacteria
some bacteria reduce nitrates to nitrites
Chemical urinalysis by reagent strip - blood - nitrite - increased sensitivity when?
Urine in bladder at least 4 hours
Chemical urinalysis by reagent strip - blood - leukocyte esterase - normal
negative
Chemical urinalysis by reagent strip - blood - leukocyte esterase - principle
LE reaction
Chemical urinalysis by reagent strip - blood - leukocyte esterase - significance
UTI
Chemical urinalysis by reagent strip - blood - leukocyte esterase - longest reaction time
2 minutes
Chemical urinalysis by reagent strip - blood - leukocyte esterase - detects
intact & lysed granulocytes and monocytes
Chemical urinalysis by reagent strip - blood - leukocyte esterase - does NOT detect?
lymphocytes
Chemical urinalysis by reagent strip - blood - specific gravity - normal
Random specimen: 1.003-1.030
Chemical urinalysis by reagent strip - blood - specific gravity - principle
pKa change of polyelectrolyte
Chemical urinalysis by reagent strip - blood - specific gravity - significance
Indication of kidney’s concentrating ability & state of hydration
-increased: DM due to glucose
-decreased: diabetes insipidus due to decreased ADH
Chemical urinalysis by reagent strip - blood - specific gravity - only measures?
ionic solute
Chemical urinalysis by reagent strip - blood - specific gravity - not affected by?
-urea
-glucose
-radiographic contrast media
-plasma expanders
General sources of error with reagent strip testing: failure to test within 2 hours of collection or to preserve correctly - possible effect?
changes in chemical composition
General sources of error with reagent strip testing: failure to bring refrigerated specimens to room temperature before testing - possible effect?
False negative enzymatic reactions
General sources of error with reagent strip testing: failure to mix specimen well- possible effect?
False negative leukocyte/blood
-WBCs, RBCs settle out
General sources of error with reagent strip testing: failure to perform quality control - possible effect?
Erroneous results
-perform pos & neg control every 24 hours & when opening new container
General sources of error with reagent strip testing: failure to dip all test pads in urine - possible effect?
False negative reactions
General sources of error with reagent strip testing: prolonged dipping - possible effect?
False negative reactions
-reagents may leach from pads
General sources of error with reagent strip testing: failure to remove excess urine from strip - possible effect?
run over of chemicals to adjacent pads, distortion of colors
General sources of error with reagent strip testing: highly pigmented urine - possible effect?
atypical colors, false positive reactions
-pigment masks true reactions
-test by alternate method
General sources of error with reagent strip testing: failure to read at recommended time - possible effect?
erroneous results
General sources of error with reagent strip testing: failure to store strips properly - possible effect?
erroneous results
-store in capped original container at RT
Specific sources of error with reagent strip testing - pH - increased or false positive
improperly preserved specimen
Specific sources of error with reagent strip testing - pH - decreased or false negative
acid run over from protein square
Specific sources of error with reagent strip testing - protein - increased or false positive
-highly buffered alkaline urine
-prolonged dipping
-contaminated container
-increased specific gravity
Specific sources of error with reagent strip testing - protein - decreased or false negative
proteins other than albumin
Specific sources of error with reagent strip testing - glucose - increased or false positive
contamination with peroxide or bleach
Specific sources of error with reagent strip testing - glucose - decreased or false negative
-unpreserved specimen
-increased ascorbic acid
-increased specific gravity
-decreased temperature
Specific sources of error with reagent strip testing - ketones - increased or false positive
-red pigments
-dyes
-some meds
Specific sources of error with reagent strip testing - ketones - decreased or false negative
-improper storage:
—acetone is volatile
—bacteria break down acetoacetic acid
Specific sources of error with reagent strip testing - blood - increased or false positive
-menstruation
-oxidizing agents
-bacterial peroxidase
Specific sources of error with reagent strip testing - blood - decreased or false negative
-increased ascorbic acid
-increased nitrite
-increased specific gravity (crenated RBCs)
-unmixed specimen
Specific sources of error with reagent strip testing - bilirubin - increased or false positive
highly pigmented urine
Specific sources of error with reagent strip testing - bilirubin - decreased or false negative
-exposure to light
-increased ascorbic acid
-increased nitrite
Specific sources of error with reagent strip testing - urobilinogen - increased or false positive
highly pigmented urine
Specific sources of error with reagent strip testing - urobilinogen - decreased or false negative
-improperly preserved specimen (oxidation to urobilin)
-formalin
Specific sources of error with reagent strip testing - nitrite - increased or false positive
-highly pigmented urine
-improperly preserved specimen (contaminating bacteria produce nitrites)
Specific sources of error with reagent strip testing - nitrite - decreased or false negative
-non-nitrate-reducing bacteria
-inadequate time in bladder
-reduction of nitrites to N2
-decreased dietary nitrate
-antibiotics
-increased ascorbic acid
-increased specific gravity
Specific sources of error with reagent strip testing - LE - increased or false positive
-highly pigmented urine
-oxidizing agents
-formalin
-nitrofurantoin
-vaginal discharge
Specific sources of error with reagent strip testing - LE - decreased or false negative
-increased glucose
-increased protein
-increased ascorbic acid
-increased specific gravity
-antibiotics
-reading too soon
Specific sources of error with reagent strip testing - SG - increased or false positive
increased protein
Specific sources of error with reagent strip testing - SG - decreased or false negative
-alkaline urine (add 0.005 if pH is 6.5 or higher; correction is made by automated readers)
Other urine chemistry tests - microalbumin - detects
albumin in low/moderate concentrations
Other urine chemistry tests - microalbumin - method(s)
-immunoassay on 24-hour urine
-albumin-to-creatinine ratio (ACR) on random sample
-dipsticks available for ACR
Other urine chemistry tests - microalbumin - not detected by?
most urine dipsticks
Other urine chemistry tests - microalbumin - concentration predictive of diabetic nephropathy
Males:
50-200 mg/24 hour OR
ACR >=2.8
Females:
50-200 mg/24 hour OR
ACR >=2.0
Other urine chemistry tests - Ictotest - detects
bilirubin
Other urine chemistry tests - Ictotest - method(s)
Diazo reagent
Other urine chemistry tests - Ictotest - sources of error - false positives
urine pigments
Other urine chemistry tests - Ictotest - sources of error - false negatives
-exposure to light
-improperly stored specimen
-increased ascorbic acid
-increased nitrite
Other urine chemistry tests - Ictotest - less affected by?
interfering substances
Sediment stains - Sternheimer-Malbin - action
delineates cell structure and contrasting colors of nucleus vs. cytoplasm
Sediment stains - Sternheimer-Malbin - function
identify WBCs, epithelial cells, and casts
Sediment stains - Oil Red O and Sudan III - action
stains triglycerides and neutral fats, cholesterol will not stain
Sediment stains - Oil Red O and Sudan III - function
ID free fat droplets and lipid-containing cells or casts
Sediment stains - Hansel stain - action
methylene blue and eosin Y stain eosinophilic granules
Sediment stains - Hansel stain - function
ID eosinophils in the urine
Epithelial cells in the urine sediment - squamous epithelial cell - description
-40-50 um
-flat
-prominent round nucleus
Epithelial cells in the urine sediment - squamous epithelial cell - origin
-lower urethra
-vagina
Epithelial cells in the urine sediment - squamous epithelial cell - clinical significance
usually none
Epithelial cells in the urine sediment - squamous epithelial cell - increased numbers usually seen from?
females
Epithelial cells in the urine sediment - squamous epithelial cell - may obscure?
RBCs & WBCs
Epithelial cells in the urine sediment - squamous epithelial cell - reduced by?
collecting midstream clean-catch specimen
Epithelial cells in the urine sediment - transitional epithelial cell - description
-20-30 um
-spherical, pear-shaped, or polyhedral
-round central nucleus
Epithelial cells in the urine sediment - transitional epithelial cell - origin
-renal pelvis
-ureters
-bladder
-upper urethra
Epithelial cells in the urine sediment - transitional epithelial cell - clinical significance
seldom significant
Epithelial cells in the urine sediment - transitional epithelial cell - may form?
syncytia (clumps)
Epithelial cells in the urine sediment - renal tubular epithelial cell - description
-slightly larger than a WBC (12 um)
-round
-eccentric round nucleus
Epithelial cells in the urine sediment - renal tubular epithelial cell - origin
renal tubules
Epithelial cells in the urine sediment - renal tubular epithelial cell - clinical significance
-tubular necrosis
-toxins
-viral infections
-renal rejection
Epithelial cells in the urine sediment - renal tubular epithelial cell - must differentiate from?
WBCs
Epithelial cells in the urine sediment - oval fat body - description
renal tubular epithelial cell containing fat droplets
Epithelial cells in the urine sediment - oval fat body - origin
renal tubules
Epithelial cells in the urine sediment - oval fat body - clinical significance
-tubular necrosis
-toxins
-viral infections
-renal rejection
Epithelial cells in the urine sediment - oval fat body - polarized light
Maltese crosses
Blood cells in the urine sediment - WBCs - description
-usually polys
-about 12 um
-granular appearance
Blood cells in the urine sediment - WBCs - origin
kidney, bladder, or urethra
Blood cells in the urine sediment - WBCs - clinical significance
-cystitis
-pyelonephritis
-tumors
-renal calculi
Blood cells in the urine sediment - WBCs - normal
0-8/HPF
Blood cells in the urine sediment - WBCs - clumps
associated with acute infection
Blood cells in the urine sediment - glitter cell - description
-WBC with Brownian movement of granules
-stain faintly or not at all
Blood cells in the urine sediment - glitter cell - description
-WBC with Brownian movement of granules
-stain faintly or not at all
Blood cells in the urine sediment - glitter cell - origin
kidney, bladder, or urethra
Blood cells in the urine sediment - glitter cell - clinical significance
-cystitis
-pyelonephritis
-tumors
-renal calculi
Blood cells in the urine sediment - glitter cell - observed in?
hypotonic urine
Blood cells in the urine sediment - RBC - description
-biconcave disk
-about 7 um
-smooth
-non-nucleated
Blood cells in the urine sediment - RBC - origin
kidney, bladder, or urethra
Blood cells in the urine sediment - RBC - clinical significance
-infection
-trauma
-tumors
-renal calculi
-dysmorphic RBCs indicate glomerular bleeding
Blood cells in the urine sediment - RBC - normal
0-3/HPF
Blood cells in the urine sediment - RBC - hypertonic urine
crenated RBCs
Blood cells in the urine sediment - RBC - hypotonic urine
RBCs lyse
Blood cells in the urine sediment - RBC - 2% acetic acid
RBCs lyse
Normal crystals found in acid or neutral urine - amorphous urates - description
irregular granules
Normal crystals found in acid or neutral urine - amorphous urates - precipitate
pink
Normal crystals found in acid or neutral urine - amorphous urates - may obscure?
significant sediment
Normal crystals found in acid or neutral urine - amorphous urates - dissolve by?
warming to 60*C
Normal crystals found in acid or neutral urine - uric acid - description
-pleomorphic
-4-sided, 6-sided, star-shaped, rosettes, spears, plates
-colorless, red-brown, or yellow
Normal crystals found in acid or neutral urine - uric acid - birefringent?
yes
Normal crystals found in acid or neutral urine - uric acid - polarizes light?
yes
Normal crystals found in acid or neutral urine - calcium oxalate - description
-octahedral (8-sided) envelope form is most common
-dumbbell & ovoid forms
Normal crystals found in acid or neutral urine - calcium oxalate - form that may be mistaken for RBCs
monohydrate form
What is the most common constituent of renal calculi?
calcium oxalate
Normal crystals found in acid or neutral urine - calcium oxalate - caused by?
oxalate-rich foods
Normal crystals found in alkaline urine - amorphous phosphates - description
irregular granules
Normal crystals found in alkaline urine - amorphous phosphates - precipitate
white
Normal crystals found in alkaline urine - amorphous phosphates - dissolves with?
2% acetic acid
Normal crystals found in alkaline urine - triple phosphate - description
“coffin-lid” crystal
Normal crystals found in alkaline urine - ammonium biurate - description
yellow-brown “thorn apples” & spheres
Normal crystals found in alkaline urine - ammonium biurate - seen in what type of urine specimens?
old specimens
Normal crystals found in alkaline urine - calcium phosphate - description
-needles
-rosettes
-“pointing finger”
Normal crystals found in alkaline urine - calcium phosphate - what form is the only form seen in alkaline urine?
needle form
Normal crystals found in alkaline urine - calcium carbonate - description
colorless dumbbells or aggregates
Abnormal crystals (acid or neutral urine) - leucine - description
-yellow, oily-looking spheres
-radial & concentric striations
Abnormal crystals (acid or neutral urine) - leucine - significance
severe liver disease
Abnormal crystals (acid or neutral urine) - leucine - often seen with what other crystal?
tyrosine
Abnormal crystals (acid or neutral urine) - tyrosine - description
fine yellow needles in sheaves or rosettes
Abnormal crystals (acid or neutral urine) - tyrosine - significance
severe liver disease
Abnormal crystals (acid or neutral urine) - tyrosine - often seen with what other crystal?
leucine
Abnormal crystals (acid or neutral urine) - cystine - description
hexagonal (6-sided)
Abnormal crystals (acid or neutral urine) - cystine - significance
cystinuria
Abnormal crystals (acid or neutral urine) - cystine - must differentiate from?
uric acid
Abnormal crystals (acid or neutral urine) - cystine - polarizes light?
no
Abnormal crystals (acid or neutral urine) - cholesterol - description
-flat plates
-notched out corners
-“stair steps”
Abnormal crystals (acid or neutral urine) - cholesterol - significance
nephrotic syndrome
Abnormal crystals (acid or neutral urine) - cholesterol - birefringent?
yes
Abnormal crystals (acid or neutral urine) - bilirubin - description
yellowish brown needles, plates, granules
Abnormal crystals (acid or neutral urine) - bilirubin - significance
liver disease
Abnormal crystals (acid or neutral urine) - bilirubin - chemical tests should be?
positive
Casts - hyaline - description
homogeneous with parallel sides, rounded ends
Casts - hyaline - normal
0-2/LPF
Casts - hyaline - significance
increased:
-stress
-fever
-trauma
-exercise
-renal disease
Casts - hyaline - type of protein?
Tamm-Horsfall
Casts - hyaline - dissolve in?
alkaline urine
Casts - hyaline - why might they be overlooked with bright light?
because they have the same refractive index as urine
Casts - granular - description
-homogeneous with parallel sides, rounded ends
-granules
Casts - granular - normal
0-1/LPF
Casts - granular - significance
increased:
-stress
-exercise
-glomerulonephritis
-pyelonephritis
Casts - granular - from disintegration of?
cellular casts
Casts - RBC - description
-RBCs in cast matrix
-yellowish to orange color
Casts - RBC - significance
-acute glomerulonephritis
-strenuous exercise
Casts - RBC - source of bleeding?
kidneys
Which urinary cast is the most fragile and often found in fragments?
RBC casts
Casts - blood - description
-contain hemoglobin
-yellowish to orange color
Casts - blood - significance
-acute glomerulonephritis
-strenuous exercise
Casts - blood - from disintegration of?
RBC casts
Casts - WBC - description
-WBCs in cast matrix
-irregular in shape
Casts - WBC - significance
pyelonephritis
Casts - WBC - IDs what as the site of infection?
kidneys
Casts - epithelial cell - description
renal tubular epithelial cells in cast matrix
Casts - epithelial cell - significance
renal tubular damage
Where are casts formed?
renal tubules & collecting ducts
Are transitional & squamous epithelial cells seen in casts?
no
Casts - waxy - description
-homogeneous
-opaque
-notched edges
-broken ends
Casts - waxy - significance
urinary stasis
Casts - waxy - from degeneration of?
cellular & granular casts
The presence of what type of urinary casts is considered an unfavorable sign?
waxy cast
Casts - fatty - description
casts containing lipid droplets
Casts - fatty - significance
nephrotic syndrome
Casts - fatty - what is seen with polarized light if the lipid inside the cast is cholesterol?
Maltese crosses
Casts - broad - description
-wide
-may be cellular, granular, or waxy
Casts - broad - significance
advanced renal disease
Casts - broad - formed in?
dilated distal tubules & collecting ducts
What type of urinary casts are considered “renal failure casts”?
broad casts
Miscellaneous urine sediment - bacteria - description
rods, cocci
Miscellaneous urine sediment - bacteria - significance
UTI or contaminants
Miscellaneous urine sediment - bacteria - what is also present if bacteria are clinically significant?
WBCS - unless patient is neutropenic
Miscellaneous urine sediment - yeast - description
-5-7 um
-ovoid
-colorless
-smooth
-refractile
-may bud & form pseudohyphae
Miscellaneous urine sediment - yeast - significance
-usually due to vaginal or fecal contamination
-may be due to kidney infection
-often in urine in diabetics
Miscellaneous urine sediment - yeast - how to differentiate from RBCs?
add 2% acetic acid - RBCs lyse; yeast do not
Miscellaneous urine sediment - yeast - pseudohyphae indicate?
severe infection
Miscellaneous urine sediment - yeast - what are also present in true yeast infections?
WBCs
Miscellaneous urine sediment - sperm - description
-4-6 um head
-40-60 um tall
Miscellaneous urine sediment - sperm - significance
-usually not significant in adults
-may be sign of sexual abuse in child
Miscellaneous urine sediment - Trichomonas - description
-resembles WBC
-flagella & undulating membrane
-rapid, jerky, non-directional motility
Miscellaneous urine sediment - Trichomonas - significance
parasitic infection of genital tract
Miscellaneous urine sediment - Trichomonas - reporting
don’t report unless motile
Miscellaneous urine sediment - Mucus - description
transparent, long, thin, ribbon-like structure with tapering ends
Miscellaneous urine sediment - Mucus - significance
none
Miscellaneous urine sediment - Mucus - may be mistaken for?
hyaline casts
Renal disorders - acute glomerulonephritis - cause
inflammation & damage to glomeruli
Renal disorders - acute glomerulonephritis - reagent strip
protein, blood
Renal disorders - acute glomerulonephritis - sediment
-RBCs (some dysmorphic)
-WBCs
-hemoglobin casts
Renal disorders - acute glomerulonephritis - frequently follows what type of infection?
group A strep infection
Renal disorders - nephrotic syndrome - cause
increased glomerular permeability
Renal disorders - nephrotic syndrome - reagent strip
protein (large amount)
Renal disorders - nephrotic syndrome - sediment
-casts (all kinds)
-free fat & oval fat bodies
Renal disorders - nephrotic syndrome - blood
-hypoproteinemia
-hyperlipidemia
Renal disorders - pyelonephritis - cause
kidney infection
Renal disorders - pyelonephritis - reagent strip
-protein
-LE
-nitrite
Renal disorders - pyelonephritis - sediment
-WBCs
-WBC casts
-bacteria
Renal disorders - cystitis - cause
bladder infection
Renal disorders - cystitis - reagent strip
-LE
-nitrite
Renal disorders - cystitis - sediment
-WBCs
-bacteria
-possibly RBCs
-NO casts
Renal disorders - acute interstitial nephritis - cause
allergic reaction to medication
Renal disorders - acute interstitial nephritis - reagent strip
-LE
-blood
-protein
Renal disorders - acute interstitial nephritis - sediment
-WBCs
-WBC casts
-RBC casts
-NO bacteria
Renal disorders - acute interstitial nephritis - frequently presents with?
rash
Renal disorders - Fanconi syndrome - cause
Failure of tubule reabsorption:
-hereditary
-exposure to drugs
-multiple myeloma
Renal disorders - Fanconi syndrome - reagent strip
-glucose
-protein
-low pH
Renal disorders - Fanconi syndrome - plasma glucose
normal
Urinalysis correlations - pH/microscopic
-check pH when identifying crystals
-RBCs, WBCs, & casts lyse at alkaline pH
Urinalysis correlations - pH/nitrite/LE/microscopic
Bacterial UTI:
-pos nitrite
-pos LE
-check for bacteria & WBCs
-bacteria convert urea to ammonia, increased pH
Urinalysis correlations - protein/microscopic
-protein may indicate renal disease
-check for casts
Urinalysis correlations - protein/blood/microscopic
-large amounts of blood or myoglobin can cause positive protein
-check for RBCs
-present with hematuria, NOT with hemoglobinuria or myoglobinuria
Urinalysis correlations - protein/SG
-increased SG may cause false positive trace protein
-trace protein is more significant in dilute urine
Urinalysis correlations - glucose/ketones
ketones present in uncontrolled DM
Urinalysis correlations - glucose/microscopic
-yeast thrives in increased glucose
-WBCs should be present if true yeast infection
Urinalysis correlations - glucose/protein/microscopic
-renal disease is common complication of DM
-check for casts
Urinalysis correlations - blood/microscopic
Pos blood, no RBCs:
-blood reaction could be due to hemoglobin or myoglobin OR
-false pos due to bacterial peroxidase
-check for bacteria
Neg blood, RBCs seen:
-could be false negative blood due to increased ascorbic acid
-yeast or monohydrate calcium oxalate crystals could be misidentified as RBCs
Urinalysis correlations - bilirubin/urobilinogen
Liver disease:
-bili pos or neg
-urobili increased
Biliary obstruction:
-bili pos
-urobili negative
Hemolytic disorder:
-bili neg
-urobili increased
Urinalysis correlations - LE/microscopic
-can have pos LE without WBCs (WBCs lysed)
-can have pos LE & WBCs without bacteria (Trichomonas)
Urinalysis correlations - Nitrite/LE/microscopic
-with Pos nitrite, usually have pos LE, WBCs, & bacteria
-can have pos LE, WBCs, bacteria, & NEG nitrite (non-nitrate-reducing bacteria)
Urinalysis correlations - SG/microscopic
-RBCs & WBCs lyse in dilute urine
-with increased SG, RBCs & RBCs may crenate, cause false-negative blood & LE
CSF - color - normal
colorless
CSF - color - abnormalities - xanthochromia
-slight pink, orange, or yellow due to oxyhemoglobin or bilirubin
-seen in subarachnoid hemorrhage
CSF - color - abnormalities - traumatic tap
red or pink, decreasing from tube 1-3
CSF - color - examine within?
1 hour of collection to avoid false positive from lysis of RBCs
CSF - clarity - normal
clear
CSF - clarity - abnormalities
cloudy with infection or bleeding
CSF - WBC - normal
Adult: 0-5/uL
Newborn: 0-30/uL
(mononuclear)
CSF - WBC - abnormalities
increased in meningitis
CSF - WBC - perform cell count within?
30 minutes of collection - cells begin to lyse within 1 hour
CSF - WBC - count done in?
hemacytometer, following lysis of RBCs with 3% glacial acetic acid
CSF - WBC - differential on stained smear following?
concentration (cytocentrifugation)
CSF - RBC - normal
0
CSF - RBC - abnormalities
increased with subarachnoid hemorrhage, traumatic tap
CSF - RBC - diagnostic value
limited
CSF - Glucose - normal
60-70% of blood glucose
CSF - Glucose - abnormalities
decreased in bacterial meningitis
CSF - Glucose - method
blood glucose method
CSF - Protein - normal
15-45 mg/dL
CSF - Protein - abnormalities
increased in meningitis & with traumatic tap
CSF - Protein - method
biuret method is not sensitive enough, use alternative method
CSF - cells - normal
-lymphocytes
-monocytes
-ependymal cells
-choroid plexus cells
CSF - cells - abnormalities
-siderophages with subarachnoid hemorrhage
-blasts with leukemia
CSF - cells - nucleated RBCs seen?
due to bone marrow contamination
Differential diagnosis of meningitis:
WBC: increased
Differential: neutrophils
Protein: increased
Glucose: decreased
Lactate: increased
Other: pos gram stain, bacterial antigen
bacterial meningitis
Differential diagnosis of meningitis:
WBC: increased
Differential: lymphocytes
Protein: increased
Glucose: normal
Lactate: normal
viral meningitis
Differential diagnosis of meningitis:
WBC: increased
Differential: lymphs, monos
Protein: increased
Glucose: decreased
Lactate: increased
Other: weblike clot or pellicle
mycobacterial meningitis
Differential diagnosis of meningitis:
WBC: increased
Differential: neutrophils
Protein: increased
Glucose: normal or decreased
Lactate: increased
Other: pos India ink and/or Cryptococcus neoformans antigen test
fungal meningitis
abnormal accumulation of fluid in body cavity; classified as transudate or exudate
effusion
fluid contained in pericardial, peritoneal, & pleural cavities
serous fluid
fluid surrounding heart
pericardial fluid (pericardiocentesis fluid)
fluid in abdominal cavity
peritoneal fluid (abdominal fluid, ascitic fluid)
fluid surrounding lungs
pleural fluid (chest fluid, thoracentesis fluid, empyema fluid)
fluid in joints
synovial fluid (joint fluid)
Differentiation of transudates and exudates - Transudate - etiology
-systemic disorder affecting fluid filtration & reabsorption (CHF, hypoalbuminemia, cirrhosis)
-problem originating outside body cavity
Differentiation of transudates and exudates - Transudate - type of process
non-inflammatory
Differentiation of transudates and exudates - Transudate - color
colorless
Differentiation of transudates and exudates - Transudate - clarity
clear
Differentiation of transudates and exudates - Transudate - SG
<1.015
Differentiation of transudates and exudates - Transudate - protein
<3 g/dL
Differentiation of transudates and exudates - Transudate - fluid-to-serum protein ratio
<0.5
Differentiation of transudates and exudates - Transudate - glucose
equal to serum level
Differentiation of transudates and exudates - Transudate - spontaneous clotting
no
Differentiation of transudates and exudates - Transudate - lactate dehydrogenase
<60% of serum
Differentiation of transudates and exudates - Transudate - WBC
<1,000/uL
Differentiation of transudates and exudates - Transudate - differential
predominantly mononuclears
Differentiation of transudates and exudates - Exudate - etiology
condition involving membranes within body cavity (infection, malignancy, inflammation, hemorrhage)
Differentiation of transudates and exudates - Exudate - type of process
inflammatory
Differentiation of transudates and exudates - Exudate - color
yellow, brown, red, green
Differentiation of transudates and exudates - Exudate - clarity
cloudy
Differentiation of transudates and exudates - Exudate - SG
> 1.015
Differentiation of transudates and exudates - Exudate - protein
> 3 g/dL
Differentiation of transudates and exudates - Exudate - fluid-to-serum protein ratio
> 0.5
Differentiation of transudates and exudates - Exudate - glucose
30 mg or more < than serum level
Differentiation of transudates and exudates - Exudate - spontaneous clotting
yes
Differentiation of transudates and exudates - Exudate - lactate dehydrogenase
> 60% of serum
Differentiation of transudates and exudates - Exudate - WBC
> 1,000/uL
Differentiation of transudates and exudates - Exudate - differential
predominantly neutrophils
Synovial fluid - normal - color
pale yellow to colorless
Synovial fluid - normal - clarity
clear
Synovial fluid - normal - viscosity
good
Synovial fluid - normal - WBcs/uL
<200
Synovial fluid - normal - Polys
<25%
Synovial fluid - non-inflammatory - etiology
degenerative joint disease
Synovial fluid - non-inflammatory - color
yellow
Synovial fluid - non-inflammatory - clarity
clear
Synovial fluid - non-inflammatory - viscosity
good
Synovial fluid - non-inflammatory - WBCs/uL
<2,000
Synovial fluid - non-inflammatory - Polys
<30%
Synovial fluid - inflammatory - etiology
-rheumatoid arthritis
-lupus erythematosus
-gout
-pseudogout
Synovial fluid - inflammatory - color
yellow
Synovial fluid - inflammatory - clarity
cloudy, turbid
Synovial fluid - inflammatory - viscosity
poor
Synovial fluid - inflammatory - WBCs/uL
2,00-100,000
Synovial fluid - inflammatory - Polys
> 50%
Synovial fluid - infectious - etiology
bacterial infection
Synovial fluid - infectious - color
yellow-green
Synovial fluid - infectious - clarity
cloudy, turbid
Synovial fluid - infectious - viscosity
poor
Synovial fluid - infectious - WBCs/uL
50,000-200,000
Synovial fluid - infectious - Polys
> 90%
Synovial fluid - infectious - other
positive culture (S. aureus, N. gonorrhoeae, most common)
Synovial fluid - crystal induced - etiology
gout, pseudogout
Synovial fluid - crystal induced - color
yellow, white
Synovial fluid - crystal induced - clarity
cloudy, milky
Synovial fluid - crystal induced - viscosity
poor
Synovial fluid - crystal induced - WBCs/uL
500-200,000
Synovial fluid - crystal induced - Polys
<90%
Synovial fluid - hemorrhagic - etiology
trauma, coagulation abnormality
Synovial fluid - hemorrhagic - etiology
trauma, coagulation abnormality
Synovial fluid - hemorrhagic - color
pink, red, red-brown
Synovial fluid - hemorrhagic - clarity
cloudy
Synovial fluid - hemorrhagic - viscosity
poor
Synovial fluid - hemorrhagic - WBCs/uL
50-10,000
Synovial fluid - hemorrhagic - Polys
<50%
Synovial fluid crystals - monosodium urate - description
-1-30 um long needles
-intra- or extracellular
-strongly birefringent
-yellow when long axis of crystal is parallel to slow wave of red compensator; blue when perpendicular
Synovial fluid crystals - monosodium urate - significance
gout
Synovial fluid crystals - calcium pyrophosphate - description
-1-20 um long, 4 um wide
-rod-shaped, rectangular, or rhomboid
-intracellular
-weakly birefringent
-blue when long axis of crystal is parallel to slow wave of red compensator; yellow when perpendicular
Synovial fluid crystals - calcium pyrophosphate - significance
pseudogout
Synovial fluid crystals - cholesterol - description
-large rectangle with notched-out corner
-extracellular
Synovial fluid crystals - cholesterol - significance
extracellular
Semen analysis - fertility testing - specimen collection
-collect in sterile container, without condom, after 3-day abstinence
-keep at RT
-deliver to lab within 1 hour of collection
Semen analysis - fertility testing - liquefaction
don’t analyze until specimen is liquefied (normally within 30 minutes of collection)
Semen analysis - fertility testing - volume
Normal: 2-5 mL
Semen analysis - fertility testing - motility
-observe within 3 hours of collection
-50-60% of sperm should show at least fair motility
Semen analysis - fertility testing - cell count
-dilute & count in Neubauer hemacytometer
-Normal: >20 million per mL
Semen analysis - fertility testing - morphology
-stain & examine at least 200 cells
-Normal: oval head (3 x 5 um) with long tapering tail
-Abnormalities: double heads, giant heads, amorphous heads, pinheads, tapering heads, constricted heads, double tails, coiled tails, large numbers of spermatids (immature forms)
-NORMAL: <=30% abnormal forms
Semen analysis - fertility testing - pH
7.3-8.3
Semen analysis - post-vasectomy - specimen collection
-condom can be used
-time & temp not critical
-test monthly beginning 2 months after vasectomy
-continue until 2 consecutive specimens are without sperm
Semen analysis - post-vasectomy - liquefaction
don’t analyze until specimen is liquefied
Semen analysis - post-vasectomy - cell count
-examine undiluted & following centrifugation
-even 1 sperm is significant
Amniotic fluid tests - Lecithin-to-sphingomyelin (L/S) ratio - assessment/diagnosis of
fetal lung maturity
Amniotic fluid tests - Lecithin-to-sphingomyelin (L/S) ratio - method
thin-layer chromatography
Amniotic fluid tests - Lecithin-to-sphingomyelin (L/S) ratio - ratio that signal maturity
> =2.0
Amniotic fluid tests - Lecithin-to-sphingomyelin (L/S) ratio - false increases
blood or meconium contamination
Amniotic fluid tests - Phosphatidylglycerol (PG) - assessment/diagnosis of
fetal lung maturity
Amniotic fluid tests - Phosphatidylglycerol (PG) - assessment/diagnosis of
fetal lung maturity
Amniotic fluid tests - Phosphatidylglycerol (PG) - method
immunologic agglutination
Amniotic fluid tests - Phosphatidylglycerol (PG) - affected by presence of blood or meconium?
no
What is the last surfactant to rise (fetal lung maturity)?
phosphatidylglycerol
Amniotic fluid tests - Foam stability index (shake test) - assessment/diagnosis of
fetal lung maturity
Amniotic fluid tests - Foam stability index (shake test) - method
shake with increasing amounts of 95% ethanol
Amniotic fluid tests - Foam stability index (shake test) - index is?
highest concentration of ethanol that supports ring of foam after shaking
Amniotic fluid tests - Foam stability index (shake test) - what index is comparable to L/S ratio of 2.0?
0.48
Amniotic fluid tests - Foam stability index (shake test) - does blood and meconium interfere?
yes
Amniotic fluid tests - lamellar body count - assessment/diagnosis of
fetal lung maturity
Amniotic fluid tests - lamellar body count - method
count in platelet channel of hematology analyzers
Amniotic fluid tests - lamellar body count - number correlates with?
amount of phospholipid present in fetal lungs
Amniotic fluid tests - lamellar body count - sample must be free of?
blood or meconium
Amniotic fluid tests - amniotic fluid bilirubin - assessment/diagnosis of
hemolytic disease of the newborn/fetus
Amniotic fluid tests - amniotic fluid bilirubin - method
direct spectrophotometric scan from 365-550 nm
Amniotic fluid tests - amniotic fluid bilirubin - peak absorbance
450 nm
Amniotic fluid tests - amniotic fluid bilirubin - determine severity of disease by?
deltaA450 compared to gestational age
Amniotic fluid tests - amniotic fluid bilirubin - specimen must be protected from?
light
Amniotic fluid tests - amniotic fluid bilirubin - do blood and meconium interfere?
yes
Amniotic fluid tests - alpha-fetoprotein (AFP) - assessment/diagnosis of
neural tube defects
Amniotic fluid tests - alpha-fetoprotein (AFP) - method
immunologic method
Amniotic fluid tests - alpha-fetoprotein (AFP) - high levels with?
-anencephaly
-spina bifida
Amniotic fluid tests - alpha-fetoprotein (AFP) - confirm with?
ultrasound
A first morning urine specimen is best for screening and for routine urinalysis because it:
A. Is produced when the body is in a resting state
B. Is more concentrated
C. Is not affected by diurnal variation
D. Will be the most acidic
B. Is more concentrated
Which of the following “color of urine: cause” is a correct match?
A. Red: concentrated specimen
B. Black: bilirubin
C. Green: Pseudomonas infection
D. Pink or red: homogentisic acid
C. Green: Pseudomonas infection
Which of the following cells found in the urine sediment is indicative of tubular damage or necrosis?
A. WBC
B. Epithelial cell
C. Renal tubular epithelial cell
D. Transitional epithelial cell
C. Renal tubular epithelial cell
In a patient with severe liver disease, which of the following crystals would be anticipated upon examination of their urine sediment?
A. leucine
B. tyrosine
C. bilirubin
D. all of the above
D. all of the above
Large amounts of protein (proteinuria) are most frequently found associated with which of the following renal diseases?
A. Nephrotic syndrome
B. Acute glomerulonephritis
C. Acute interstitial nephritis
D. Cystitis
A. Nephrotic syndrome
In which of the following conditions would a positive leukocyte esterase reagent strip in the absence of bacteria is most likely?
A. Acute pyelonephritis
B. Trichomonas infection
C. Cystitis
D. All of the above
B. Trichomonas infection
Which of the following characterizes a subarachnoid hemorrhage rather than a traumatic spinal tap?
A. Increase in protein
B. Clear supernatant after centrifugation
C. Xanthochromia
D. Presence of bacteria
C. Xanthochromia
Which of the following laboratory results is characteristic of an exudate fluid?
A. Clear and colorless
B. Total protein = 2.5 g/dL
C. Fluid/serum protein ratio = 0.3
D. Specific gravity = 1.021
D. Specific gravity = 1.021
In which form of meningitis is a normal CSF glucose value most often observed?
A. Bacteria
B. Fungal
C. Viral
D. Mycobacterial
C. Viral
Which of the following amniotic fluid tests is utilized for the diagnosis of open neural tube defect?
A. Lamellar body count
B. AFP
C. Bilirubin
D. Phosphatidylglycerol
B. AFP