Resolutions

Question 1

Fluorescence Imaging

Answer 1

200-300nm

Question 2

Light microscopy

Answer 2

Around 200nm spatial

Question 3

Confocal microscopy

Answer 3

Lateral Resolution ~200–300 nm
Axial Resolution ~500–700 nm

Question 4

Near-field super-resolution microscopy

Answer 4

<20nm spatial

Question 5

Structural Illumination Microscopy (SIM) and Saturated Structural Illumination Microscopy (SSIM).

Answer 5

SIM (100nm) and SSIM (50nm)

Question 6

STORM (Stochastic Optical Reconstruction Microscopy) and PALM (Photoactivated Localisation Microscopy).

Answer 6

20nm spatial.

Question 7

EM

Answer 7

0.5-1nm (SEM & TEM)
TEM spacings (10-20nm)
CryoFIB 200-300nm slice

Question 8

OCT

Answer 8

1-10µm axial with penetration of 1-3mm

Question 9

Film X-Ray

Answer 9

16 lp/mm (16 line pairs per millimetre - per mm can resolve 16 alternating lines)

AKA: ~31 µm spatial

Question 10

Digital X-ray

Answer 10

3.5-5.5 lp/mm but some 22 lp/mm

AKA (80–100 µm spatial detail)

Question 11

CT

Answer 11

Body is imaged in 0.05-30mm thick slices, and temporal resolution is 75-250ms.

Question 12

fMRI

Answer 12

2mm spatial

Temporal Resolution ~1–3 seconds per volume

Question 13

DCE-MRI

Answer 13

Temp around 10s

Question 14

PET

Answer 14

2-9mm around 30sec