Resolution and Detection of Nucleic Acids Flashcards
1
Q
Electrophoresis
A
Movement of molecules by an electric current.
2
Q
Anode
A
Positive pole
3
Q
Pulsed-field Gel Electrophoresis
A
Pulses of current applied to the gel in alternating dimensions to enhance the migration for large molecules.
4
Q
Field-inversion Gel Electrophoresis
A
Alternating positve and negative electrodes during electrophoresis.
5
Q
Denaturing Agents
A
Break hydrogen bonds between complementary strands. Formamide or Urea.
6
Q
pH Drift
A
Decrease of pH at the cathode and an increase of pH at the anode.
7
Q
Submarine Gels
A
Horizontal gels. Gels that are submerged with buffer.
8
Q
TAE
A
Tris Acetate EDTA buffer
9
Q
TBE
A
Tris borate EDTA buffer
