Nucleic Acid Extraction Methods Flashcards
1
Q
Buffer
A
An ionic substance or solution that maintains specific pH.
2
Q
Immobilized
A
Bound to a substrate such as nitrocellulose-based membrane or glass slide
3
Q
RNase Free
A
RNF. Reagents, consumables, and facilities prepared or treated to protect RNA from RNA digesting proteins.
4
Q
Intracellular
A
Inside of the cell
5
Q
Microfuge
A
A small centrifuge designed to accommodate.2-2.0ml tubes.
6
Q
Silver stain
A
A sensitive staining system for nucleic acids and proteins after gel electrophoresis.
7
Q
Absorpitivity Constants
A
The characteristic tendency of a substance to absorb light at a given wavelength, used in quantifying nucleic acids, the absorpitivity constants of DNA and RNA are (50ug/ml)/absorbance unit and (40ug/ml)/absorbance unit, respectively, at 260nm wavelength.
