Resistant pathogens

Question 1

How do pathogens become resistance?

Answer 1

1. Pathogen population had varying ability to resist drug as some are susceptible and some resistant.
2. Drug kills most susceptible pathogens first.
3. Remaining population becomes resistant.
4. Exposure to sub-lethal dose of medication allows bacteria to develope resistance by chance mutations.
5. Over time, resistance of population increases

Question 2

What is resistance to drugs?

Answer 2

Selection pressure

Question 3

What is aseptic technique?

Answer 3

The practice of preventing contamination of cultures by unwanted microorganisms

Question 4

What are examples of aseptic technique when culturing bacteria ?

Answer 4

Working near open flame to create zone of sterile air, heat treating metal items to sterilise, open bottles at 45 degrees to reduce SA for contamination, flame neck of bottle to expand air around and prevent spores from falling in

Question 5

What are examples of aseptic technique after creating bacteria plata?

Answer 5

Sellotape with 2 short strips and not whole way around, incubate below body temperature and autoclave petri dishes

Question 6

Why do you sellotape the petri dish with 2 short strips?

Answer 6

Prevent anaerobic bacteria from growing which is very hazardous and ensure enough oxygen for bacteria to survive

Question 7

Why do you incubate below body temperature?

Answer 7

To prevent the mutation of bacteria which can survive in the human body and divide

Question 8

Why do you autoclave and dispose petri dishes?

Answer 8

To sterilise petri dish and kill any bacteria to prevent spread