Research Methods Flashcards
What is immunohistochemistry?
It’s when an antibody for a protein/peptide hormone is bound to a fluorescent dye, and it’s used to identify hormone producing cells.
What is autoradiography?
It’s when a tissue is injected with radioactively labelled hormone. Used to determine where there’s larger concentrations of hormone (darker regions)
What’s immunological neutralization of hormone activity?
It’s when an antibody is prepared against a peptide hormone and the antibody neutralizes the biological activity of the endocrine hormone.
What are bioassays used for?
They’re used to study the actions of a hormone on living cells.
What does Actinomycine D do?
It inhibit transcription
What is the direct ELISA procedure?
First, a known antibody is added to a plate. The sample suspected of containing a specific antigen is then added. Next, an enzyme-linked antibody is added. If the antigen is present in the well, the enzyme-linked antibody will bind to the antigen. Then, a colourless substrate is added to the well. If a colour appears, it is evident that the antigen is present.
What is Western Blotting?
It’s a rapid test, which separates proteins according to their size. It’s incubated with a primary antibody, and detected with a secondary antibody. (Heavier proteins are closer to the top, and lighter proteins migrate further)
What’s radioimmunoassay?
It’s a technique used to determine the concentration levels of the hormone in the blood. (A known concentration of radioactive hormone, and its antibody are added to the mixture, and the ratio of bound hormone to unbound hormone is used to determine the concentration of free hormone – by comparing it to a standard binding curve made experimentally)
How is insulin produced today to treat patients with Type 1 diabetes?
Produced by bacteria.
What is Polymerase Chain Reaction?
In vitro amplification of specific DNA sequences
