Research Methods

Question 1

Lesioning and microinjection

Answer 1

Electrolytic lesions or neurotoxin

Lesions can damage pathways

Question 2

Microdialysis

Answer 2

Using cannula, collect NTs from specific brain areas and use HPLD to separate molecules and determine concentration

Question 3

Electrophysiology

Answer 3

Stimulation and recording of neurons via electrodes, invented by neher and sakmann

Question 4

Radio ligand binding

Answer 4

Measures quantity of receptors using gamma counter from homogenate
Must be:
1. Specific- measure with high affinity non radioactive ligand, measure remaining radioactivity (unbound)
2. Saturability
3. Reversibility and high affinity
4. Biological relevance-correlates with measurable effect in vivo

Question 5

Receptor autoradiography

Answer 5

Location of receptors

Question 6

Immunocytochemistry

Answer 6

Locate specific molecules within cells

Question 7

In situ hybridization

Answer 7

Locate mRNA by using a fluorescent probe

In slice or homogenate

Question 8

Construct validity

Answer 8

Extent to which test accurately measures characteristic being investigated

Question 9

Face validity

Answer 9

Extent to which tests on animals can be generalized to humans

Question 10

Predictive validity

Answer 10

extent to which lab test can predict clinical effects

Question 11

Tail flick test

Answer 11

Latency to move tail when heat applied as measure of analgesia

Question 12

Self administration test

Answer 12

Animals have access to a lever which they press to release the drug. Addiction measured by seeing e level a pain an animal will withstand to obtain the drug. Higher breaking point=larger reinforcement

Question 13

Stereotactic surgery

Answer 13

Implantation of a device using stereotactic atlas based on bregma