Reproduction In Plants Flashcards
Whats vegetative propagation?
Type of asexual reproduction in which new plants develop from vegetative part of plant.
Concept of tissue culture was given by
Haberlandt.(1901)
Whats tissue culture?
Growth of explant in vitro conditions.
Growth of cells, tissues, organs in lab conditions.
Whats explant?
Cells, tissue or organs that is used to start culture technique.
Loading of explant over nutrient medium is called
Innoculation.
Closed chamber used to conduct tissue culture is c/d
Bio-reactor or innoculation chamber.
Nutrient medium should have
Minerals
Sucrose (c12h22o11)(glu-glu)
Phytohormones esp. auxin and cytokinins
Most commonly used nutrient medium is
MS medium.
Whats liquid medium?
Prepared by dissolving all contents with water.
Aka suspension medium.
Whats solid/gel medium?
Prepared by adding 1-2% agar-agar in liquid medium.
Sterilisation is done by
Boiling or treatment with alcohol.
Surface sterilisation is done by
Adding anti-microbials like chlorax.
UV sterilisation is done by
Passing uv radiations in a bio-reactor.
Whats continuous culture?
When older medium is replaced by new fresh medium.
Whats batch culture?
When cells are shifted to new medium.
Nucellus culture is done so as to
Produce disease free plants.
Give 2 significance of embryo culture?
To overcome problem of seed dormancy.
To perform embryo rescue (to prevent embryo from forming non-viable seeds).
Production of virus free plants is obtained by ______ culture.
Meristem culture.
Whats androgenic haploid?
Haploid plants developed from anther cells(male).
Which type of tissue culture results in production of androgenic haploids?
Anther culture.
Whats cybrid?
Cybrid / somatic hybrid =organism produced by crossing two genetically organisms asexually.
Whats micropropagation /somo-clonal propagation?
Type of tissue culture in which a new plant is produced from small group of cells.
Production of cybrid is a significance of _________ culture.
Protoplast culture (fused protoplast is used as explant).
Virus used in protoplast culture for fusion of protoplast of 2 cells is ______ .
Sendai virus (used in viral fusion of protoplast).
PEG stands for _______ used in _______fusion of protoplast.
Poly ethylene glycol used in chemo-fusion of protoplasts of 2 cells during protoplast culture.
Whats artificial seed?
Embryoids (aka somatic embryo, is a embryo like structure produced during tissue culture) wrapped in gelatin or agar agar represent artificial seeds.
Whats cutting?conditions for cutting?
Formation of new plant from ‘detached part of plant’ under suitable conditions.
Conditions:
1. Presence of at-least one bud.
2.abundance of meristematic tissue.
3. Suitable conditions; phytohormones like auxin, ethylene.
Examples of root cutting?
Sweet potato
Asparagus
Dahlia
sad
Examples of leaf cutting?
Bryophyllum
Begonia
Examples of stem cutting?
Grapes
Rose
Apple
Whats layering?
Formation of new plant from ‘attached part of plant’ under suitable conditions.
Layering examples ?
Jasmine
Blackberry
Walnut (Air layering)
Grapes
Whats Grafting ?
Union of 2 plant parts to form a composite plant.
OR
Union of scion and stock to form composite plant.
Define scion & stock?
Plant part used along with root system = stock
Branch shifted over stock = scion
Grafting is not possible in monocots due to absence of vascular cambium.
(T/F)
True.
Naturally vegetative propagation by roots is seen in
Sweet potato
Asparagus
Dahlia
Naturally vegetative propagation by leaf is seen in
Bryophyllum
Begonia
Sansveria (snake plant)
Kalonchi
Naturally vegetative propagation by buds either bulbils or turions is seen in
Bulbils: Agave, lily, ananas,oxalis.
Turions: utricularia.
Naturally vegetative propagation by aerial stem is observed in
Cactus (opuntia) = flat phylloclade
Euphorbia = cylindrical phylloclade
Which organism can participate in vegetative propagation?
Marchantia , a liverwort(belonging to bryophyta) possess green multicellular asexual buds c/d gemma which develops inside gemma cups.
Vegetative propagation in lawn grass occurs by
Runner =a sub-aerial stem
Jasmine & peppermint perform vegetative propagation by
Stolon = a sub-aerial stem
Water hyacinth(eichorrnia) & water lettuce show vegetative propagation by
Offset =sub-aerial stem
Sucker (sub-aerial stem) is involved in vegetative propagation in
Banana
Pineapple
Chrysanthemum
pcb
Potato shows vegetative propagation by
Tuber = underground stem